Photoprotein derived from okinawan squid and gene encoding...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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Details

C435S252300, C435S252800, C435S243000, C435S320100, C435S069100, C435S191000, C544S346000, C536S023100

Reexamination Certificate

active

10835180

ABSTRACT:
By using a bioluminescence, a method for detection of a monovalent cation is provided and the method is a high sensitive and simple method. According to the present invention, an amino acid sequence of symplectin and a base sequence encoding the protein are provided. Symplectin is a photoprotein derived fromSymplectoteuthis oualaniensis(okinawan squid). By using the photoprotein of the present invention, a monovalant cation can be detected by luminescence in the presence of a chromophore.

REFERENCES:
Guo et al. , PNAS 101,9205-9210 (2004).
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M. Isobe et al., “Synthesis of 13C-Dehydrocoelenterazine and model studies onsymplectoteuthissquid bioluminescence”, Bioorganic and Medicinal Chemistry Letters, vol. 8, 1998, pp. 2919-2924.
H. Takahashi et al., “Photoprotein of luminous squid,symplectoteuthis oualaniensisand reconstruction of the luminous system”, Chemistry Letters, 1994, pp. 843-846.
F.I. Tsuji et al., “K+ / Na+ -triggered bioluminescence in the oceanic squidsymplectoteuthis oualaniensis”, Proc. Nat. Acad. Sci. USA, vol. 78, No. 11, 1981, pp. 6719-6723.
M. Isobe et al., “Molecular mechanisms of bioluminescence of symplectin, a photoprotein from Okinawan squid,symplectoteuthis ouelaniensis L.” Tennen Yuki Kagobutsu Toronkai Koen Yoshishu, vol. 42nd., 2000, pp. 97-102.
M. Isobe et al., “Chemistry of photoproteins as interface between bioactive molecules and protein function” Pure & Appl. Chemistry, vol. 70, No. 11, 1998, pp. 2085-2092.
Shevchenko, Andrej, et al., “Rapid ‘de Novo’ Peptide Sequencing by a Combination of Nanoelectrospray, Isotopic Labeling and a Quadrupole/Time-of-flight Mass Spectrometer”Rapid Communications in Mass Spectrometry.1997. pp. 1015-1024. vol. 2. John Wiley & Sons, Ltd.

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