Photon counting fluorimeter

Optics: measuring and testing – By dispersed light spectroscopy – With sample excitation

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Details

250458, 250459, 356323, 356417, G01N 2164

Patent

active

043209700

ABSTRACT:
An ultraviolet light beam is split into two beams which are directed toward a reference cell and a sample cell, respectively. A mechanical rotating light chopper permits the reference and sample cells to be alternately illuminated. Radiation from the reference and sample cells is directed toward and sensed by a single photomultiplier tube. An up/down counter counts the number of photons impinging on the photomultiplier tube from the reference and sample cells. Photodiodes in conjunction with the mechanical light chopper cause the up/down counter to count up when photons are arriving from the sample cell and cause the up/down counter to count down when photons are arriving from the reference cell. A source intensity monitor prohibits further measurements after a preselected amount of incident flux has been sensed. The intensity monitor includes a photodetector circuit which senses a portion of the incident light. Pulses from the detector are fed to a counter and the number in the counter is compared to a preselected number which is set on a thumbwheel switch. When the comparison condition is met, the up/down counter of the fluorimeter is stopped.

REFERENCES:
patent: 3738755 (1973-06-01), Chaney et al.
patent: 3832555 (1974-08-01), Ohnishi
patent: 3854050 (1974-12-01), Peterson et al.
patent: 3886363 (1975-05-01), Ohnishi et al.
patent: 3897155 (1975-07-01), Smythe
patent: 3903422 (1975-09-01), Buhrer

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