Photometric determination of protein, and of endotoxin with Limu

Details Photometric determination of protein, and of endotoxin with Limu Photometric determination of protein, and of endotoxin with Limu

1977-11-04

1978-08-01

Marantz, Sidney

Chemistry: physical processes

Physical processes

Crystallization

1951035R, 252408, 260112R, 424 2, G01N 3316, G01N 2106

Patent

active

041040309

ABSTRACT:
An optical density technique for measuring protein concentration generally, or for measuring minute traces of endotoxin in an unknown material by the clotting of Limulus lysate protein. After the clotting step, the clotted protein is separated and digested in from 0.1 to 0.4N strong alkali such as sodium hydroxide and then photometrically analyzed, for example with the Lowry test. Greatly improved stability of a quantitative endopoint is achieved under this circumstance.

REFERENCES:
patent: 2897058 (1959-07-01), Goldt
patent: 3558278 (1971-01-01), Louderback
patent: 3873272 (1975-03-01), Wakefield
patent: 3884637 (1975-05-01), Gindler
patent: 3915805 (1975-10-01), Levin
patent: 3944391 (1976-03-01), Harris
patent: 3954663 (1976-05-01), Yamamoto
patent: 4023933 (1977-05-01), Bradford
patent: 4038029 (1977-07-01), Teller
patent: 4038147 (1977-07-01), Reno
R. Nandan et al., J. Lab. Clin. Med., 89 (4), 910-918, Apr. 1977.
Chemical Abstracts, 84:145808p (1976).
Chemical Abstracts, 84:155680e (1976).

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