Photometric calibration of liquid volumes

Details Photometric calibration of liquid volumes Photometric calibration of liquid volumes

2007-03-06

2007-03-06

Lauchman, Layla G. (Department: 2877)

Optics: measuring and testing

Dimension

Volume

C356S436000

Reexamination Certificate

active

10827611

ABSTRACT:
A method and apparatus for measuring and calibrating the measurement of small volumes of liquids. The small volumes of liquid are typically dispensed from liquid delivery devices, the delivery device often having multiple channels to analyze many samples at once. The liquid samples are delivered to one or more cells, typically in a multi-well plate, and positioned in a spectrophotometer for determining an absorbance of a chromophore in the liquid sample. Based upon an absorbance measurement and the concentration of the chromophore, a path length of the liquid sample is determined, from which a volume of the sample may be calculated. The method and apparatus provide various means for correcting for differences in the dimensions and/or other factors causing a non-linear deviation from the Beer-Lambert law. A system or kit may be provided including sets of sample solutions of varying dilution ranges for calibrating different liquid volumes. The kit may further include software code for storing and analyzing the various sample solutions.

REFERENCES:
patent: 4354376 (1982-10-01), Greenfield et al.
patent: 5244813 (1993-09-01), Walt et al.
patent: 5258308 (1993-11-01), Freeman et al.
patent: 5298978 (1994-03-01), Curtis et al.
patent: 5492673 (1996-02-01), Curtis et al.
patent: 5766875 (1998-06-01), Hafeman et al.
patent: 5959738 (1999-09-01), Hafeman et al.
patent: 5963318 (1999-10-01), Held
patent: 6188476 (2001-02-01), Hafeman et al.
patent: 6320662 (2001-11-01), Hafeman et al.
patent: 6339472 (2002-01-01), Hafeman et al.
patent: 6741365 (2004-05-01), Curtis
patent: 2002/0149772 (2002-10-01), Halg

Affiliated with

Also associated with

Rating

Photometric calibration of liquid volumes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Photometric calibration of liquid volumes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Photometric calibration of liquid volumes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3776363