Optics: measuring and testing – Blood analysis – Hemoglobin concentration
Patent
1989-09-26
1991-11-12
McGraw, Vincent P.
Optics: measuring and testing
Blood analysis
Hemoglobin concentration
356246, G01N 3349, G01N 2103
Patent
active
050642820
ABSTRACT:
An apparatus and method for making a photometric absorbance measurement of hemoglobin in a blood sample utilizing a reusable cuvette and an inexpensive photometer. The cuvette is a unitary plastic body having a flow-through slot of a predetermined height which allows an absorbance measurement to be made with an undiluted blood sample and which permits cleaning by inserting the body in a liquid cleaning solution. Two absorbance measurements are made, the first at a wavelength at which the absorbance of oxyhemoglobin and deoxyhemoglobin are approximately equal, near an isobestic point, and a second measurement at which these components absorb substantially no light. By making the first measurement near an absorbance minimum at about 510 nm, an absorbance measurement can be made with a relatively thick slot required for cleaning and with an undiluted blood sample and an inexpensive photometer.
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Von Schneck et al., "Evaluation of HemoCue, A New Device For Determining Hemoglobin", Clinical Chemistry, vol. 32, No. 3 (Mar. 1986).
Artel, Inc.
McGraw Vincent P.
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