Photobleaching by high power pulses

Details Photobleaching by high power pulses Photobleaching by high power pulses

2007-10-19

2010-02-09

Evans, F. L (Department: 2877)

Optics: measuring and testing

By dispersed light spectroscopy

With raman type light scattering

C356S036000

Reexamination Certificate

active

07659978

ABSTRACT:
A pulse photobleaching methodology wherein a monochromatic illumination (e.g., laser illumination) having a higher power intensity (photobleaching power) just below the photodamage threshold of a luminescent sample is initially used to significantly attenuate sample luminescence without photothermally destroying the sample material. Thereafter, the laser power density may be reduced to a significantly lower level (analytical power level) to carry out spectroscopic measurements (e.g., collection of Raman scattered photons) on the sample. In one embodiment, the laser illumination wavelength remains the same despite changes in laser power intensity. Some figures-of-merit may be computed from optical measurements made at the analytical power level to guide the photobleaching process. Sample-dependent combinations of laser power density and short exposure times may be obtained to significantly expedite photobleaching to assist in collection of sample spectral data in the field without a long wait. Portable spectroscopy systems employing pulse photobleaching may be devised for expeditious collection of spectral data from luminescent samples in the field.

REFERENCES:
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patent: 2006/0092414 (2006-05-01), Geshwind et al.
patent: 2006/0200013 (2006-09-01), Smith et al.
patent: 2006/0203238 (2006-09-01), Gardner et al.
MacDonald et al., On the Use of Photobleaching to reduce Fluorescence Background in Raman Spectroscopy to Improve the Reliability of Pigment Identification on Painted Textiles, J. Raman Spec., vol. 37, p. 830-835 (Aug. 2006).
Rosch et al., Chemotaxonomic Identification of Single Bacteria by Micro-Raman Spectroscopy: Application to Clean-Room Relevant Biological Contaminations, Appl. Env. Microbiol., vol. 71, No. 3, p. 1626-1637 (Mar. 2005).

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