Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or... – Inactivation or attenuation; producing viral subunits
Patent
1995-06-07
1998-07-14
Degen, Nancy
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
Inactivation or attenuation; producing viral subunits
536 231, C12N 704, C12N 706, C07H 2104
Patent
active
057802874
ABSTRACT:
Disclosed herein are compounds, compositions, and methods to inactivate a virus and destroy tumor cells. The methods involve the addition into the cell of a compound containing a photosensitizing chemical and an energy donating chemical, optionally linked by a chemical tether. Also introduced into the cell are means to chemically activate the energy donating chemical which photoactivates the photosensitizing chemical which then destroys the tumor or virus. The photosensitizing chemical is preferably hypericin, porphyrin, or an analog and the energy donating chemical is preferably luciferin or an analog. Methods for synthesizing the chemicals are also disclosed. Further, the energy donating chemical is activated by an activating chemical. The expression of the activating chemical is regulated so as to target the virus-infected or tumor cells. Regulating the activating chemical is accomplished by a number of methods including construction of an expression plasmid containing a gene encoding the activating chemical under control of a promoter which is transactivated by replication of the virus or transactivated by elevated levels of proteins expressed in tumor cells.
REFERENCES:
patent: 4898891 (1990-02-01), Lavie et al.
patent: 5047435 (1991-09-01), Lavie et al.
patent: 5049589 (1991-09-01), Lavie et al.
White, et al. 1975. J. of the American Chemical Society. vol. 97, No. 5 pp. 1243-1245.
Liebes, et. al. 1991. Anal. Biochemistry. vol. 195, pp. 77-85.
Campbell, et al. 1985. in Methods of Biochemical Analysis, Glick, ed. Wiley and Sons. especially pp. 340-345.
Schwartz, et al. 1990. Gene, vol. 88 pp. 197-205.
Kraus, G. A. et. al. 1990. Biochemical and Biophysical Research Communications, pp. 149-153. vol. 172, No. 1.
Rodriguez, et al. 1988. PNAS, USA vol. 85 pp. 1667-1671.
Lenard, et al., "Photodynamic inactivation of infectivity of human immunodeficiency virus and other enveloped viruses using hypericin and rose bengal: Inhibition of fusion and syncytia formation" Proc. Natl. Acad. Sci, USA, 90:158-162 (Jan. 1993)).
Van Steveninck et al., "Photodynamic Effects Induced by the Luciferin/Luciferase System," Photochemistry and Photobiology, 43:2, 213-216 (1986).
Carpenter et al., "Identification of a Hypervariable Region in the Long Terminal Repeat of Equine Infectious Anemia Virus," Journal of Virology, 65:3, 1605-1610 (Mar. 1991).
Kraus et al., "Antiretroviral Activity of Synthetic Hypericin and Related Analogs," Biochemical and Biophysical Research Communications, 172:149, 153 (Oct. 15, 1990).
Carpenter et al., "Photosensitization is Required for Inactivation of Equine Infectious Anemia Virus by Hypericin," Photochemistry and Photobiology, 53:2, 169-174 (1991).
John S. James, "Hypericin Update," AIDS Treatment News, 125:4-6 (Apr. 19, 1991).
John S. James, Hypericin, Feb. 1992, AIDS Treatment News 146:1-4 (Mar. 6, 1992).
Meruelo et al., "Therapeutic agents with dramatic antiretroviral activity and little toxicity at effective doses: Aromatic polycylic diones hypericin and pseudohypericin," Proc. Natl. Sci. USA, 85:5230-5234 (Jul. 1988).
Matthews et al., "Photodynamic therapy of viral contaminants with potential for blood banking applications," Transfusion, 28:1, 81-83 (1988).
Lavie et al., "Studies of the mechanisms of action of the anitiretroviral agents hypericin and pseudohypericin," Proc. Natl. Acad. Sci. USA, 86:5963-5967 (Aug. 1989).
Chanh et al., "Photodynamic liactivation of simian liunodeficiency virus," Journal of Virological Methods, 26:125-132 (1989).
Moan, J., "Yearly Review: Porphyrin Photosensitization and Phototherapy", Photochemistry and Photobiology, 43:6, 681-690 (1986).
"Luciferase Assay System," Promega Technical Bulletin, 101:12/.90.
"GeneLight.TM. Plasmids Technical Maual," Promega, #TM003, Oct. 1991.
Hudson et al., "Antiviral activities of hypericin," Antiviral Research, vol. 15, pp. 101-112 (1991).
Thomas et al., "Oxygen Dependance of Hypericin-induced Phototoxicity to EMT6 Mouse Mammary Carcinoma Cells", Photochemistry and Photobiology, vol. 55, No. 6, pp. 831-837, 1992.
Culver et al., Lymphocytes as cellular vehicles for gene therapy in mouse and man, Proc. Natl. Acad. Sci. USA, vol. 88, pp. 3155-3159, Apr. 1991.
Cornetta et al., Protamine sulfate as an effective alternative to polybrene in retroviral-mediated gene-transfer: implications for human gene therapy, Journal of Virological Methods, 23 (1989) 187-194.
Kantoff et al., Expression of Human Adenosine Deaminase in Nonhuman Primates After Retrovirus-Mediated Gene Transfer, The Rockefeller University Press, vol. 166, pp. 219-234, Jul. 1987.
Kasid et al., Human gene transfer: Characterization of human tumor-infiltrating lymphocytes as vehicles for retroviral-mediated gene transfer in man, Proc. Natl. Acad. Sci, USA, vol. 87, pp. 473-477, Jan. 1990.
Kantoff et al., Correction of adenosine deaminase deficiency in cultured human T and B cells by retrovirus-mediated gene transfer, Proc. Natl. Acad. Sci. USA, vol. 83, pp. 6563-6567, Sep. 1986.
Lim et al., Retrovirus-Mediated Gene Transfer of Human Adenosine Deaminase: Expression of Functional Enzyme in Murine Hematopoietic Stem Cells in Vivo, Molecular and Cellular Biology, Oct. 1987, pp. 3459-3465 (1987).
Introduction of Recombinant Vectors into Mammalian Cells, Expression of Cloned Genes in Cultured Mammalian Cells.
Eli Gilboa, Retroviral Gene Transfer: Application to Human Therapy, Retroviruses and Disease, 1989.
Carpenter Susan L.
Kraus George A.
Petrich Jacob W.
Degen Nancy
Iowa State University Research Foundation
LandOfFree
Photoactivated antiviral and antitumor compositions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Photoactivated antiviral and antitumor compositions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Photoactivated antiviral and antitumor compositions will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1880871