Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-20
1996-06-18
Rees, Dianne
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 911, 536 221, 536 231, 536 2721, 536 2854, 536 285, C12Q 168, C12Q 170, C12P 1934, C07H 1904
Patent
active
055276830
ABSTRACT:
The present invention discloses a photo-induced DNA-cleaving agent composition comprises N-aryl-N-(alkyl or arylalkyl)hydroxylamine having the following formula: ##STR1## wherein R is C.sub.1 -C.sub.6 alkyl, phenyl, C.sub.1 -C.sub.6 alkoxy, phenoxy, C.sub.1 -C.sub.6 alkoxycarbonyl, halogen or halo(C.sub.1 -C.sub.6 alkyl)wherein R.sub.1 is hydrogen, C.sub.1 -C.sub.6 alkyl, phenyl, C.sub.1 -C.sub.6 alkoxy, C.sub.1 -C.sub.6 alkoxycarbonyl, halogen or halo(C.sub.1 -C.sub.6 alkyl); R.sub.2 is hydrogen; R.sub.3 is hydrogen or phenyl; R.sub.4 is hydrogen, phenyl, hydroxylphenyl, methoxyphenyl, dimethoxyphenyl, dimethylaminophenyl or naphthyl. The present N-aryl-N-(alkyl or arylalkyl)hydroxylamine is stable in dark, but it can react with O.sub.2 to form HO. radicals under irradiation of UV light for a period of 2-3 hours. The HO. radicals then react with DNA to accomplish cleavage of DNA.
REFERENCES:
patent: 4797350 (1989-01-01), Ohbayashi et al.
Vaught et al. "Arylhydroxylamine--induced ribonucleic acid chain cleavage ad chromatographic analysis of arylamine ribonucleic acid adducts" Chem. Biol. Interactions 34: 109-124. 1981.
Sternson et al. Reaction of Phenylhydroxylamine with Bisulfite. A possible model for Amine-medicated carcinogenesis. J.Org. Chem 48: 57-60. 1983.
Jih Ru Hwu, et al., "N--Arylalkyl--N--phenylhydroxylamines as Novel Photo-induced DNA--cleaving Agents", J. Chem. Soc., Chem. Commun., Jun. 1994.
Chen Buh-Luen
Chen Wan-Lin
Chou Ching-Tai
Hwu Jih R.
Lin Chun C.
National Science Council
Rees Dianne
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