Phosphorylating reagents

Details Phosphorylating reagents Phosphorylating reagents

1993-02-26

1994-07-26

Brown, Johnnie R.

Organic compounds -- part of the class 532-570 series

Organic compounds

Acyclic carbon bonded directly to three benzene rings or to...

544157, C07F 909, C07F 9141

Patent

active

053328450

ABSTRACT:
Novel phosphorylating reagents are provided which are useful in DNA synthesis and purification procedures. The reagents are substituted phosphines which are particularly useful in the phosphorylation of nucleoside and solid supported oligonucleotides at the 5'-hydroxyl position. Phosphorylation using these reagents is easily and accurately monitored colorimetrically.

REFERENCES:
patent: 4415732 (1983-11-01), Caruthers et al.
patent: 4692542 (1987-09-01), Tesser et al.
patent: 5039796 (1991-08-01), Engels et al.
Claesen et al. Recl. Trav. Chim. 104: 119-122, 1985.
Fisher et al. Nucl. Acids Res. 11(5): 1589-1599, 1983.
Beaucage et al., Tetrahedron Letters, 22(20): 1859-1862, 1981.
Froehler et al., Tetrahedron Letters, 24(31): 3171-3174, 1983.
Froehler et al., Nucleic Acids Res., 14(13): 5399-5407, 1986.
Froehler et al., Nucleic Acids Research, 11(22): 8031-8036, 1983.
Horn et al., Nucleosides & Nucleotides, 5(1&2): 335-340, 1987.
Horn et al., Tetrahedron Letters, 27(39): 4705-4708, 1986.
Horn et al., DNA, 5(5): 421-426, 1986.
Jablonski et al. Nucleic Acids Research 14(15): 6115-6128, 1986.
McBride et al., Tetrahedron Letters, 24(3): 245-248, 1983.
Perich et al., Tetrahedron Letters, 28(1): 101-102, 1987.
Smith, Synthesis pp. 15-24, Dec., 1983.
Sonveaux, Bioorganic Chemistry, 14: 274-325, 1986.
Uhlmann et al., Tetrahedron Letters, 27(9): 1023-1026, 1986.
Warner et al., DNA 3(5): 401-411, 1984.

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