Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Milk or colostrum
Patent
1995-11-06
1999-08-24
Railey, II, Johnny F.
Drug, bio-affecting and body treating compositions
Extract, body fluid, or cellular material of undetermined...
Milk or colostrum
426580, 530350, 530360, 530832, A61K 3520, A61K 3817, A23C 915, A23J 120
Patent
active
059422548
ABSTRACT:
The present invention provides a plasmid containing a promoter sequence, a nucleotide sequence encoding an exogenous protein, and a nucleotide sequence encoding an enzyme capable of modifying the exogenous protein. In a specific embodiment of the invention the encoded exogenous protein is human .beta.-casein and the encoded enzyme is a human kinase capable of phosphorylating recombinant .beta.-casein in a bacterial system. Phosphorylated recombinant human .beta.-casein synthesized using the plasmid of the invention is shown to have the same bioactivity as native human .beta.-casein.
REFERENCES:
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309 Journal of Dairy Science (ADSA), 71, (1988) Oct., No. 10, Champaign, IL, USA. Jimeneze-Flores, R. et al., "Genetic engineering of the caseins to modify the behavior of milk during processing: A review".
Chemical Abstracts, vol. 120, No. 3, Jan. 17, 1994, Columbus, OH, US, Abstract 25819, Hansson, Lennart et al., "Expression of human milk.beta.-casein in Escherichia coli . . . ".
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Biochemical and Biophysical Research Communications, vol. 203, No. 1, Aug. 30, 1994, pp. 225-230, Jeng et al., "Production of an Enzymatically Active E1 Component of Human Pyruvate Dehydrogenase Complex in Escherichia coli: Supporting Role of E1 Beta Subunit in E1 Activity".
Proceedings of the National Academy of Sciences, USA, vol. 91, Mar. 15, 1994, pp. 2767-2771, Shi et al., "Expression of Recombinant Human Casein Kinase II and Recombinant Heat Shock Protein 90 in Excherichia coli and Characterization of Their Interactions".
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Baxter Jeffrey Harris
Hansson Lennart
Hards Robert George
Mukerji Pradip
Thurmond Jennifer Marie
Abbott Laboratories
Becker Cheryl L.
Railey II Johnny F.
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