Phospholipid affinity purification of Factor VIII:C

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530413, 530415, 424101, C07K 318, C07K 320, C07K 1514

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active

047958065

ABSTRACT:
Affinity purification of Factor VIII:C, both plasma derived and genetically engineered, using coupled phosphatidylserine (PS) as the predominant phospholipid (PH) results in a high degree of purity of Factor VIII:C, similar to that previously demonstrated with monoclonal antibodies specific to either Factor VIII:C or von Willebrand factor. Phospholipids that can be used in combination with PS are phosphatidycholine (PC) and phosphatidylethanolamine (PE).

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patent: 4675385 (1987-06-01), Herring
Andersson et al., Thromb. Res., 23, 481-89, 1981.
Andersson et al., Biochem. J., 200, 161-167, 1981.

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