Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1995-07-26
1998-05-05
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
536 236, 536 231, 435198, 435197, 435195, 435 691, 4353201, 435 9153, C12N 120, C12N 920, C07H 2104, C12P 2106
Patent
active
057473277
DESCRIPTION:
BRIEF SUMMARY
TECHNICAL FIELD
The present invention relates to a phospholipase D gene originated from a plant.
BACKGROUND ART
Phospholipase D (hereinafter also referred to as "PLD") is one of phospholipid-decomposing enzymes, which catalyzes, for example, the reaction of decomposing lecithin to liberate phosphatidic acid and choline. This enzyme is known to occur in plants, animals and microorganisms. PLD is used as a reagent for measuring phospholipids in blood, as well as for hydrolysis of phospholipids and for production of derivatives by a base-exchange reaction utilizing the reversible reaction by PLD.
Purification and partial purification of PLDs originated from plants have been reported. That is, purification of rice PLD (Men Hui Lee, 1989, Phospholipase D of rice bran, I, purification and characterization, Plant Science 59, 25-33); partial purification of rice PLD (Katsumi TAKANO, Ikuzo KAMOI and Tetsujiro OBARA, 1987, About separation and purification, and properties of rice bran phospholipase D, J. Jpn. Soc. Food Sci. Technol., 34, 8-13 (1987)); purification of peanuts PLD (Michael Heller, Nava Mozes, Irena Peri and Eddie Maes, 1974, Phospholipase D from peanut seeds, IV, Final purification and some properties of the enzyme, Biochem. Biophys, Acta 369, 397-410); and purification of cabbage PLD (Romy Lambrecht and Renate Ulbrich-Hofmann, 1992, A facile purification procedure of phospholipase D from cabbage and its characterization, Biol. Chem. Hoppeseyler 373(2), 81-88) have been reported. However, amino acid sequences of plant PLDs have not been reported at all and genetical analyses thereof have also not been reported.
On the other hand, analyses of PLD genes of microorganisms and animals have been reported (Japanese Laid-open Patent Application (Kokai) No. 3-187382; Adrian L. M. Hodgson, Phillip Bird, and Ian T. Nisbet 1990, Cloning, nucleotide sequence, and expression in Escherichia coli of the phospholipase D gene from Corynebacterium pseudotuberculosis, Journal of Bacteriology 172, 1256-1261; and Japanese Laid-open Patent Application (Kokai) No. 5-76357).
If a PLD gene originated from a plant were available, the PLD originated from the plant may be produced in a large scale by a genetic engineering process, which is industrially advantageous. However, as mentioned above, since the amino acid sequence and DNA sequence of plant PLD have not been reported, it has hitherto been impossible to genetically manipulate the PLD gene. Further, as mentioned above, although the amino acid sequences and DNA sequences of PLDs of microorganisms and animals have been reported, since homologies of sequences are not observed among the PLD genes of microorganisms or among the PLD genes of microorganisms and animals, it is difficult to isolate plant PLD gene based on the reported information. Further, since there is no information about plant PLD gene, an antisense DNA which suppresses expression of the plant PLD gene has not been obtained.
DISCLOSURE OF THE INVENTION
An object of the present invention is to provide a PLD gene originated from a plant. Another object of the present invention is to provide an antisense DNA which can suppress the expression of the above-mentioned PLD gene according to the present invention. Still another object of the present invention is to provide a DNA which regulates expression of the PLD gene originated from a plant.
After intensive study, the present inventors succeeded in isolation of rice PLD gene and in sequencing the PLD gene by purifying PLD from rice, determining the partial amino acid sequence thereof, screening a rice cDNA library using as a probe the PCR product obtained by using the oligonucleotides encoding the above-mentioned partial amino acid sequence, cloning the inserted gene of positive clones and sequencing the inserted gene. Further, the present inventors succeeded in obtaining a cDNA clone of maize PLD using as a probe the DNA encoding rice PLD, and in sequencing the maize PLD gene. The present inventors still further succeeded in isolating a genome DNA clone car
REFERENCES:
Hayashi et al, Derwent WPI, EP 504869 (Sep. 23, 1992).
Derwent WPI, JP 04222527 (Aug. 12, 1992).
Wang et al. (1944). J. Biol. Chem. 269(32): 20312-20317 Aug. 12, 1994.
Takano et al, J. Jpn. Soc. Food Sci. Technol., vol. 34, No. 1, pp. 8-13 (1987).
Lee, Plant Science, vol. 59, pp. 25-33 (1989).
Brauer et al, Plant Physiol., vol. 92, pp. 672-678 (1990).
Abousalham et al, Biochimica et Biophysica Acta, vol. 1158, pp. 1-7 (1993).
Annual Mtg. of the American Society of Plant Physiologists, Pittsburgh, PA, Aug. 1-5, 1992, Abstract 447.
J. Biochem. 83, 677-680 (1978).
The Journal of Biological Chemistry, vol. 252, No. 3 pp. 1102-1106, 1977.
Plant Science, 59 (1989) 25-33.
Biochimica et Biophysica Acta, 369 (1974) 397-410.
Nippon Shokuhin Kogyo Gakkaishi vol. 34, No. 1, 8-13 (1987).
Journal of Bacteriology, Mar. 1990, pp. 1256-1261, vol. 172, No. 3.
Lambrecht, Romy and Ulbrich-Hofmann, Renate. Fachbereich Biochemie/Biotechnologie, Institut fur Biochemie, vol. 373 (1992).
Morioka Shinji
Ueki Jun
Japan Tobacco Inc.
Saidha Tekchand
Wax Robert A.
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