Phosphoamidase assay

Details Phosphoamidase assay Phosphoamidase assay

2003-10-10

2008-03-25

Weber, Jon (Department: 1657)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S007720

Reexamination Certificate

active

07348160

ABSTRACT:
The present invention relates to the use of compounds and to methods for the qualitative and/or quantitative determination of the activity of phosphoamidases or protein phosphoamidases specific for hydrolyzing phosphoamide (P—N) bonds N-phosphorylated basic amino acids like phospholysine, phosphoarginine and phosphohistidine.

REFERENCES:
patent: 6812015 (2004-11-01), Klumpp et al.
Mountfort et al. Evaluation of the Fluorometric Protein Phosphatase Inhibition Assay in the Determination of Okadaic Acid in Mussels; Toxicon, vol. 37 (1999) pp. 909-922.
Kim et al. Protein Phosphatases 1, 2A, and 2C are Protein Histidine Phosphatases; The Journal of Biological Chemistry, vol. 268, No. 25 (1993) pp. 18513-18518.
IUBMB Enzyme Nomenclature, EC 3.9.1.1 (1961)□□http://www.chem.qmul.ac.uk/iubmb/enzyme/EC3/9/1/1.html.
Hiraishi et al. Bovine Liver Phosphoamidase as a Protein Histidine /Lysine Phosphatase; J. Biochem, vol. 126 (1999) pp. 368-374.
Ek et al. Identification and Characterization of a Mammalian 14-kDa Phosphohistidine Phosphatase; European Journal of Biochemistry, vol. 269 (2002) pp. 5016-5023.

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