Phosphite dehydrogenase mutants for nicotinamide cofactor...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Oxidoreductase

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S440000, C435S069100, C435S071100, C435S320100, C435S252300, C435S004000, C435S006120, C536S023200

Reexamination Certificate

active

07402419

ABSTRACT:
Phosphite dehydrogenase mutant enzymes were generated that provide relaxed cofactor specificity and increased thermostability over the wild type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.

REFERENCES:
patent: 6242233 (2001-06-01), Hoshino et al.
Branden et al. Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Woodyer et al. Biochemistry. Oct. 14, 2003;42(40):11604-14.
Grant et al., (1990) “Differential plasmid rescue from transgenic mouse DNAs intoEscherichia colimethylation-restriction mutants”Proc. Natl. Acad. Sci. U S A.87 (12): 4645-9.
Hofmann et al., (1999) The PROSITE Database, its status in 1999.Nucleic Acids Res.27(1): 215-9.
Schink and Friedrich (2000) Phosphite oxidation by sulphate reduction.Nature. 406(6791): 37.
Kennedy and Thompsoon (1970) Phosphonolipids: localization in surface membranes ofTetrahymena. Science168(934): 989-91.
Kulakova et al., (1997) Cloning of the phosphonoacetate hydrolase gene fromPseudomonas fluorescens23F encoding a new type of carbon-phosphorus bond cleaving enzyme and its expression inEscherichia coliandPseudomonas putida. Gene (195(1): 49-53.
Tishkov et al., (1996) Site-directed mutagenesis of the formate dehydrogenase active centre: role of the His332-Gln313 pair in enzyme catalysisFEBS Lett.390(1): 104-8.
Garcia Costas, Amaya M., et. al. (2001) “Purification and Characterization of a Novel Phosphorus-oxidizing Enzyme fromPseudomonas stutzeriWM88”The Journal of Biological Chemistry276: 17429-17436.
Metcalf, William W. and Wolfe, Ralph S. (1998) “Molecular Genetic Analysis of Phosphite and Hypophosphite Oxidation byPseudomonas stutzeriWM88”Journal of Bacteriology180, 21: 5547-5558.
Vrtis, Jennifer M. et. al. (2001) “Phosphite Dehydrogenase: An Unusual Phosphoryl Transfer Reaction”J. Am. Chem. Soc.123: 2672-2673.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Phosphite dehydrogenase mutants for nicotinamide cofactor... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Phosphite dehydrogenase mutants for nicotinamide cofactor..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Phosphite dehydrogenase mutants for nicotinamide cofactor... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3972995

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.