Phosphinothricin-resistance gene, and its use

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound

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Other Related Categories

435128, 435193, 4351723, 4352404, 4352523, 536 232, 536 237, C12P 1300, C12N 1500, C12N 500, C12N 1531, C12N 910

Type

Patent

Status

active

Patent number

052738949

Description

ABSTRACT:
Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

REFERENCES:
Dodds, Plant Genetic Engineering, Cambridge University Press, Cambridge, 1985, pp. 1-3.
Jones, in Dodds, Plant Genetic Engineering, Cambridge University Press, Cambridge, 1985, pp. 269-295.
Gasser et al; Science 244: 1293 (1989).
Vaeck et al; Ciba-Geigy-UCLA Symposium, Tamarron Colo. (1987), Plant Gene Systems and Their Biology, vol. 62: pp. 171 and 174-181.
Wohlleben et al; Gene 70: 25 (1988).
Shah et al; Science 233: 478 (1986).

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