Phosphinothricin-resistance gene, and its use

Details Phosphinothricin-resistance gene, and its use Phosphinothricin-resistance gene, and its use

1991-11-20

1993-12-28

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing nitrogen-containing organic compound

435128, 435193, 4351723, 4352404, 4352523, 536 232, 536 237, C12P 1300, C12N 1500, C12N 500, C12N 1531, C12N 910

Patent

active

052738949

ABSTRACT:
Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

REFERENCES:
Dodds, Plant Genetic Engineering, Cambridge University Press, Cambridge, 1985, pp. 1-3.
Jones, in Dodds, Plant Genetic Engineering, Cambridge University Press, Cambridge, 1985, pp. 269-295.
Gasser et al; Science 244: 1293 (1989).
Vaeck et al; Ciba-Geigy-UCLA Symposium, Tamarron Colo. (1987), Plant Gene Systems and Their Biology, vol. 62: pp. 171 and 174-181.
Wohlleben et al; Gene 70: 25 (1988).
Shah et al; Science 233: 478 (1986).

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