Phosphinothricin-resistance gene, and its use

Details Phosphinothricin-resistance gene, and its use Phosphinothricin-resistance gene, and its use

1995-06-07

1997-06-10

Martinell, James

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

C12N 1505, C12N 1500

Patent

active

056374895

ABSTRACT:
Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.

REFERENCES:
patent: 5273894 (1993-12-01), Strauch et al.
Vaeck et al., UCLA Symposium on Molecular and Cellular Biology New Series, vol. 62, Plant Gene Systems and Their Biology, pp. 171-181, Alan R. Liss, Inc., (1987).
Wohlleben et al., Gene 70:25-37 (1988).
Shah et al., Science 233:478-481 (Jul. 25, 1986).
Dodds, Plant Genetic Engineering, Cambridge University Press, Cambridge, pp. 1-3, 1985.
Jones, In Dodds, Plant Genetic Engineering, Cambridge University Press, Cambridge, pp. 269-295, 1985.
Gasser et al., Science 244:1293-1299 (1989).

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