Phosphatases with improved phytase activity

Details Phosphatases with improved phytase activity Phosphatases with improved phytase activity

2005-12-13

2005-12-13

Monshipouri, Maryam (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S320100, C435S325000, C435S006120, C435S196000, C536S023200

Reexamination Certificate

active

06974690

ABSTRACT:
The present invention provides phosphatases with improved phytase activity. The invention provides proteolytic fragments of phosphatase having improved phytase activity. A recombinant gene encoding a phosphatase fragment having improved phytase activity is also provided. The invention also includes a method of increasing the phytase activity of phosphatase by treating the phosphatase with a protease. In addition, the invention provides a new phosphatase, AppA2, having improved properties.

REFERENCES:
patent: 3819528 (1974-06-01), Berry
patent: 3860484 (1975-01-01), O'Malley
patent: 3966971 (1976-06-01), Morehouse et al.
patent: 4038140 (1977-07-01), Jaworek et al.
patent: 4375514 (1983-03-01), Siewert et al.
patent: 4460683 (1984-07-01), Gloger et al.
patent: 4470968 (1984-09-01), Mitra et al.
patent: 4734283 (1988-03-01), Siren
patent: 4765994 (1988-08-01), Holmgren
patent: 4778761 (1988-10-01), Miyanohara et al.
patent: 4914029 (1990-04-01), Caransa et al.
patent: 4915960 (1990-04-01), Holmgren
patent: 4950609 (1990-08-01), Tischer et al.
patent: 4997767 (1991-03-01), Nozaki et al.
patent: 5024941 (1991-06-01), Maine et al.
patent: 5200399 (1993-04-01), Wettlaufer et al.
patent: 5268273 (1993-12-01), Buckholz
patent: 5290765 (1994-03-01), Wettlaufer et al.
patent: 5316770 (1994-05-01), Edwards, Jr.
patent: 5318903 (1994-06-01), Bewert et al.
patent: 5366736 (1994-11-01), Edwards, Jr.
patent: 5436156 (1995-07-01), VanGorcom et al.
patent: 5443979 (1995-08-01), Vanderbeke et al.
patent: 5480790 (1996-01-01), Tischer et al.
patent: 5492821 (1996-02-01), Callstrom et al.
patent: 5516525 (1996-05-01), Edwards, Jr.
patent: 5554399 (1996-09-01), Vanderbeke et al.
patent: 5556771 (1996-09-01), Shen et al.
patent: 5612055 (1997-03-01), Bedford et al.
patent: 5691154 (1997-11-01), Callstrom et al.
patent: 5716655 (1998-02-01), Hamstra et al.
patent: 5736625 (1998-04-01), Callstrom et al.
patent: 5780292 (1998-07-01), Nevalainen et al.
patent: 5827709 (1998-10-01), Barendse et al.
patent: 5830696 (1998-11-01), Short
patent: 5830733 (1998-11-01), Nevalainen et al.
patent: 5834286 (1998-11-01), Nevalainen et al.
patent: 5853779 (1998-12-01), Takebe et al.
patent: 5863533 (1999-01-01), Van Gorcom et al.
patent: 5876997 (1999-03-01), Kretz
patent: 5891708 (1999-04-01), Saniez et al.
patent: 5902615 (1999-05-01), Saniez et al.
patent: 5935624 (1999-08-01), DeLuca et al.
patent: 5955448 (1999-09-01), Colaco et al.
patent: 5972669 (1999-10-01), Harz et al.
patent: 5985605 (1999-11-01), Cheng et al.
patent: 5989600 (1999-11-01), Nielsen et al.
patent: 6022555 (2000-02-01), DeLuca et al.
patent: 6039942 (2000-03-01), Lassen et al.
patent: 6063431 (2000-05-01), Bae et al.
patent: 6083541 (2000-07-01), Hamstra et al.
patent: 6110719 (2000-08-01), Kretz
patent: 6139892 (2000-10-01), Fredlund et al.
patent: 6139902 (2000-10-01), Kondo et al.
patent: 6140077 (2000-10-01), Nakamura et al.
patent: 6183740 (2001-02-01), Short et al.
patent: 6190897 (2001-02-01), Kretz
patent: 6204012 (2001-03-01), Hellmuth et al.
patent: 6261592 (2001-07-01), Nagashima et al.
patent: 6264946 (2001-07-01), Müllertz et al.
patent: 6274178 (2001-08-01), Beven et al.
patent: 6277623 (2001-08-01), Oh et al.
patent: 6284502 (2001-09-01), Maenz et al.
patent: 6720014 (2004-04-01), Short et al.
patent: 2001/0018197 (2001-08-01), Wong et al.
patent: 2001/0029042 (2001-10-01), Fouache et al.
patent: 0 420 358 (1991-04-01), None
patent: 0 449 376 (1991-10-01), None
patent: 0 556 883 (1993-08-01), None
patent: 0 649 600 (1995-04-01), None
patent: 0 684 313 (1995-11-01), None
patent: 0 699 762 (1996-03-01), None
patent: 0 772 978 (1997-05-01), None
patent: 0 779 037 (1997-06-01), None
patent: 0 897 010 (1999-02-01), None
patent: 0 897 985 (1999-02-01), None
patent: 0 909 821 (1999-04-01), None
patent: 0 925 723 (1999-06-01), None
patent: 0 955 362 (1999-11-01), None
patent: 0 960 934 (1999-12-01), None
patent: 2 286 396 (1995-08-01), None
patent: 2 316 082 (1998-02-01), None
patent: WO 90/05182 (1990-05-01), None
patent: WO 91/05053 (1991-04-01), None
patent: WO 91/14773 (1991-10-01), None
patent: WO 91/14782 (1991-10-01), None
patent: WO 93/14645 (1993-08-01), None
patent: WO 93/16175 (1993-08-01), None
patent: WO 93/19759 (1993-10-01), None
patent: WO 94/03072 (1994-02-01), None
patent: WO 94/03612 (1994-02-01), None
patent: WO 97/16076 (1997-05-01), None
patent: WO 97/35017 (1997-09-01), None
patent: WO 97/39638 (1997-10-01), None
patent: WO 97/45009 (1997-12-01), None
patent: WO 97/48812 (1997-12-01), None
patent: WO 98/05785 (1998-02-01), None
patent: WO 98/06856 (1998-02-01), None
patent: WO 98/20139 (1998-05-01), None
patent: WO 98/30681 (1998-07-01), None
patent: WO 98/44125 (1998-10-01), None
patent: WO 98/54980 (1998-12-01), None
patent: WO 99/08539 (1999-02-01), None
patent: WO 99/49740 (1999-10-01), None
patent: WO 00/10404 (2000-03-01), None
patent: WO 00/20569 (2000-04-01), None
patent: WO 00/41509 (2000-07-01), None
patent: WO 00/47060 (2000-08-01), None
patent: WO 00/71728 (2000-11-01), None
patent: WO 00/72700 (2000-12-01), None
patent: WO 01/58275 (2001-08-01), None
patent: WO 01/58276 (2001-08-01), None
Ostanin et al., J.B.C., 267(32), 22830-22836, Nov. 1992.
ATCC catalog for Yeasts, 19thedition, 1995.
Dassa et al., “Identification of the GeneappAfor the Acid Phosphatase (pH Optimum 2.5) ofEscherichia coli,” Mol. Gen. Genet., 200:68-73 (1985).
Piddington et al., “The Cloning and Sequencing of the Genes Encoding Phytase (phy) and pH 2.5-Optimum Acid Phosphatase (aph) fromAspergillus nigervar.awamori,” Gene, 133:55-62 (1993).
Jia et al., “Purification, Crystallization and Preliminary X-ray Analysis ofEscherichia coliPhytase,”Acta Cryst., D54:647-649 (1998).
Kim et al., “Cloning of the Thermostable Phytase Gene (phy) fromBacillussp. DS11. and its Overexpression inEscherichia coli,” FEMS Microbiology Letters, 162:185-191 (1998).
Kerovuo et al., “Isolation Characterization, Molecular Gene Cloning, and Sequencing of a Novel Phytase fromBacillus subtilis,” Applied and Environmental Microbiology, 64(6):2079-2085 (1998).
Lei et al., “Biotechnological Developments of Effective Phytases for Mineral Nutrition and Environmental Protection,”Appl. Microb. Biotech. 57(4):474-481 (2001).
Lei et al., “Nutritional Benefits of Phytase and Dietary Determinants of Its Efficacy,”J. Appl. Anim. Res. 17:97-112 (2000).
Rodriguez et al., “Different Sensitivity of RecombinantAspergillus nigerPhytase (r-PhyA) andEscherichia colipH 2.5 Acid Phosphatase (r-AppA) to Trypsin and Pepsin in Vitro,” Archives of Biochemistry and Biophysics 365(2):262-267 (1999).
Chiarugi et al., “Differential Role of Four Cysteines on the Activity of a Low M Phosphotyrosine Protein Phosphatase,” FEBS Letters 310(1):9-12 (1992).
Lim et al., “Crystal Structure ofEscherichia coliPhytase and its Complex with Phytate,” Nature Structural Biology 7(2): 108-113 (2000).
Lim et al., “Studies of Reaction Kinetics in Relation to the Tgof Polymers in Frozen Model Systems,” in Levine, eds., Water Relationships in Food, New York.NY:Plenum Press, pp. 103-122 (1991).
Boctor et al., “Enhancement of the Stability of Thrombin by Polyols: Microcalorimetric Studies,” J. Pharm. Pharmcol., 44:600-603 (1992).
Lozano et al., “Influence of Polyhydroxylic Cosolvents on Papain Thermostability,” Enzyme Microb. Technol., 15:868-873 (1993).
Touati et al., “Pleiotropic Mutations inappRReduce pH 2.5 Acid Phosphatase Expression and Restore Succinate Utilisation in CRP-deficient Strains ofEscherichia coli,” Mol. Gen. Genet. 202:257-264 (1986).
Sidhu et al., “Analysis of α-Factor Secretion Signals by Fusing with Acid Phosphatase of Yeast,”Gene54:175-184 (1987).
Zvonok et al., “Construction of VersatileEscherichia coli-Yeast Shuttle Vectors for Promoter Testing inSaccharomyces cerevisiae,” Gene66(2):313-318 (1988).
Dassa et al., “The Complete Nucleotide Sequence of

Affiliated with

Also associated with

Rating

Phosphatases with improved phytase activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Phosphatases with improved phytase activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Phosphatases with improved phytase activity will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3504276