PhO81 promotor of Saccharomyces cerevisiae and use thereof for h

Details PhO81 promotor of Saccharomyces cerevisiae and use thereof for h PhO81 promotor of Saccharomyces cerevisiae and use thereof for h

1986-08-13

1989-06-06

Teskin, Robin

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 91, 4351723, 435255, 435256, 435320, 536 27, 935 28, 935 37, 935 43, 935 56, 935 60, 935 65, 935 64, C12P 2100, C12P 2102, C12N 1500, C07H 1512

Patent

active

048371475

ABSTRACT:
The present invention relates to a DNA fragment having a promoter activity of PHO81 gene regulating the production of phosphatase, and which is obtainable from Saccharomyces cerevisiae.; a DNA fragment bearing a structural gene downstream from the above PHO81 promoter; a transformant containing a DNA fragment bearing the above PHO81 promoter; a transformant containing a DNA fragment bearing the above PHO81 promoter and a structural gene downstream from the PHO81 promoter; and a process for obtaining a gene product which includes culturing a transformant containing a DNA fragment bearing the above PHO81 promoter and a structural gene located downstream therefrom in a suitable medium until the gene product is formed and recovering the gene product from the culture. Pharmacologically important proteinous materials may be efficiently produced with the use of the above-described novel and potent promoter obtained from yeast which is a eukaryotic microorganism.

REFERENCES:
Kramer et al., Proc. Natl. Acad. Sci. vol. 81 (2) pp. 367-370 "Regulated expression of a human interferon gene on yeast: control by phosphate concentration or temperature".
Valenzuela et al., Nature vol. 298 Jul. 22 1982 pp. 317-350 "Synthesis and Assembly of hepatitis B Virus Surface Antigen particles on yeast."
Lemire et al., Mol Cell Biol. vol. 5 (8) Aug. 1985 pp. 2131-2141 "Regulation of repressible acid phosphatase gene transcription in Saccharomyces cerevisiae ".
Toh-e et al., (including Oshima) Hakko Kogaku Kaishi, vol. 61 (1) pp. 19-30 1983.
MGG. volume 162, pp. 139-149 (1978).
Oshima et al.; the Molecular Biology of the Yeast Saccharomyces, Metabolism and Gene Expression pp. 159-180(1982), Cold Spring Harbor Lab.
Kaneko et al.; Molecular and Cellular Biology 5, 248-252, Jan. (1985).
Oshima et al.; Yeast Genetics and Molecular Biology News Japan No. 18, pp. 37 Sep. 18-20, 1985.
Oshima et al.; the 57th Annual Meeting of the Genetics Society of Japan, p. 51 (C 09) Oct. 13-15, 1985.
Oshima et al.; the Japanese Journal of Genetics vol. 60 p. 644 No. 217 Dec. (1985).

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