Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1991-03-13
1993-03-30
Moskowitz, Margaret
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 2372, 435 6, C07H 1512
Patent
active
051985438
ABSTRACT:
An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a .phi.29-type DNA polymerase.
REFERENCES:
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Bernad Antonio
Blanco Luis
Salas Margarita
Consejo Superior Investigaciones Cientificas
Moskowitz Margaret
Zitomer Stephanie W.
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