Pheromone - inducible yeast promoter

Details Pheromone - inducible yeast promoter Pheromone - inducible yeast promoter

1988-06-24

1991-11-05

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 699, 435 711, 435 91, 435121, 4351721, 4351723, 435256, 4353201, 435940, 536 27, 935 6, 935 8, 935 9, 935 10, 935 22, 935 33, 935 34, 935 37, 935 47, 935 52, 935 59, 935 69, C12N 119, C12N 1531, C12N 1581

Patent

active

050631543

ABSTRACT:
A yeast promoter inducible by the appropriate pheromone and a method of expressing a gene of interest in substantial quantities by placing it under the control of the inducible promoter. DNA encoding a protein of interest is fused or linked to a pheromone - inducible yeast promoter, such as the FUSI or the FUS2 promoter, and the fusion is inserted onto a high copy vector; the resulting product is introduced into wild type yeast cells. Stimulation of these yeast cells by the appropriate pheromone results in induction of transcription of the yeast promoter and expression of the protein of interest in substantial quantities.

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