Phenylalanine dehydrogenase production

Details Phenylalanine dehydrogenase production Phenylalanine dehydrogenase production

1996-03-20

1998-02-03

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435108, 435189, 4352532, 435872, C12N 906, C12P 1322

Patent

active

057143641

ABSTRACT:
A method for producing in culture phenylalanine dehydrogenase at yields of 50+ units of enzyme per litre of culture, one unit measured as the amount required to convert one micro-mole of phenylalanine to phenyl pyrovic acid at 25.degree. C. and pH 10.8, in a growth medium comprising a nitrogen source, a buffer, phenylalanine or phenylpyruvic acid or a salt thereof at 20 mM and optionally a vitamin solution. Using phenylalanine at about 100 mM gives yields of phenylalanine dehydrogenase enzyme of 350+ units per liter of culture.

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Brearley et al., "Large-Scale Production and Purification of L-Phenylalanine Dehydrogenase from Nocardia SP.239," Separations for Biotechnology 3, Third International Symposium of the Society of Chemical Industry:79-85 (Sep. 1994).
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