Chemistry: molecular biology and microbiology – Vector – per se
Patent
1997-08-15
1999-11-02
Achutamurthy, Ponnathapu
Chemistry: molecular biology and microbiology
Vector, per se
435 6, 435 32, 435 911, 435 912, 435199, 514 44, 536 231, 536 232, 536 245, 424 932, C12N 1552, C12N 1574, A61K 3170, C07H 2100
Patent
active
059768746
ABSTRACT:
External guide sequences ("EGS") can be used to promote RNAase P-mediated cleavage of RNA transcribed from plasmids and other genetic elements which confer drug resistance on bacterial cells. Such cleavage can render the bacteria drug sensitive. In a preferred embodiment, a vector encoding an EGS is administered to an animal or human harboring antibiotic resistant bacterial cells such that the EGS is expressed in the bacterial cells, the EGS promotes RNAase P-mediated cleavage of RNA involved in conferring antibiotic resistance to the cells, and the cells are rendered antibiotic sensitive. A preferred form of administration is via inoculation of the animal or human with cells containing genes for appropriate EGSs on promiscuous plasmids. These plasmids will spread quickly through the antibiotic-resistant population of bacterial cells, thereby making the cells susceptible to antibiotic therapy.
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Altman Sidney
Guerrier-Takada Cecilia
Achutamurthy Ponnathapu
Moore William W.
Yale University
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