Phenotypic conversion of drug-resistant bacteria to drug-sensiti

Chemistry: molecular biology and microbiology – Vector – per se

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435 6, 435 32, 435 911, 435 912, 435199, 514 44, 536 231, 536 232, 536 245, 424 932, C12N 1552, C12N 1574, A61K 3170, C07H 2100

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059768746

ABSTRACT:
External guide sequences ("EGS") can be used to promote RNAase P-mediated cleavage of RNA transcribed from plasmids and other genetic elements which confer drug resistance on bacterial cells. Such cleavage can render the bacteria drug sensitive. In a preferred embodiment, a vector encoding an EGS is administered to an animal or human harboring antibiotic resistant bacterial cells such that the EGS is expressed in the bacterial cells, the EGS promotes RNAase P-mediated cleavage of RNA involved in conferring antibiotic resistance to the cells, and the cells are rendered antibiotic sensitive. A preferred form of administration is via inoculation of the animal or human with cells containing genes for appropriate EGSs on promiscuous plasmids. These plasmids will spread quickly through the antibiotic-resistant population of bacterial cells, thereby making the cells susceptible to antibiotic therapy.

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