Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-10-16
2000-04-11
Fredman, Jeffrey
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 691, 4351723, 4352402, 4353201, 536 231, 536 243, 536 2431, 530350, 436501, C12Q 168, C12N 1563, C07H 2102, C12P 2100
Patent
active
060486937
ABSTRACT:
A method of screening for a compound that affects mammalian cell cycle regulatory proteins, comprising (A) administering a compound to a cell line, wherein the cell line comprises genetic information comprising (1) a reporter gene operably linked to a gene expression control sequence, wherein the gene expression control sequence comprises an upstream activation sequence and a promoter, and the upstream activation sequence comprises a DNA region that binds to a transcription control factor that is regulated through phosphorylation by a cyclin/CDK phosphorylation system; and (2) a hybrid gene comprising a first coding region from a gene native to the cell line and a second coding region from a second gene, wherein the first gene encodes a gene product that affects phosphorylation by the cyclin/CDK phosphorylation system, and the second gene is mammalian and is homologous to the native gene, and the hybrid gene provides a gene product effective to permit normal cyclin/CDK regulation of the transcription control factor; and (B) analyzing expression of the reporter gene in the cell line, thereby determining whether the compound affects normal regulation. Specific cell lines and methods are also part of the present invention.
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BitTech, Inc.
Fredman Jeffrey
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