Phenol oxidizing enzymes

Details Phenol oxidizing enzymes Phenol oxidizing enzymes

2007-01-09

2007-01-09

Nashed, Nashaat T. (Department: 1656)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

C435S069100, C435S252300, C435S320100

Reexamination Certificate

active

10997247

ABSTRACT:
Disclosed herein are novel phenol oxidizing enzymes naturally-produced by strains of the speciesStachybotryswhich possess a pH optima in the alkaline range and which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genusStachybotrys, designated hereinStachybotrys parvisporaMUCL 38996 andStachybotrys chartarumMUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes.Disclosed herein is the amino acid and nucleic acid sequence forStachybotrysphenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression hosts.

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Koikeda, S. et al., 1993, “Molecular cloning of the gene for bilirubin oxidase fromMyrothecium verrucafiaand its expression in yeast,” Journal of Biological Chemistry, vol. 268, No. 25, pp. 18801-18809.

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