Phencyclidine and phencyclidine metabolites assay, tracers, immu

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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530362, 530363, 530367, 530386, 530395, 530404, 530405, 530408, 530409, 530807, C07K 1514, C07K 1516, C07K 1706

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active

051552124

ABSTRACT:
The present invention is directed to a fluorescence polarization assay for phencyclidine and phencyclidine derivatives, to the various components needed for preparting and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them, and a reagent kit containing them. The tracers and the immunogens are made from substituted phencyclidine compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

REFERENCES:
patent: 4196185 (1980-04-01), Focella et al.
patent: 4255329 (1981-03-01), Ullman
patent: 4281065 (1981-07-01), Lin et al.
patent: 4351760 (1982-09-01), Khanna et al.
patent: 4420568 (1983-12-01), Wang et al.
patent: 4446065 (1984-05-01), Lin et al.
patent: 4476228 (1984-10-01), Huckzermeir et al.
patent: 4476229 (1984-10-01), Fino et al.
patent: 4481136 (1984-11-01), Khanna et al.
patent: 4492762 (1985-01-01), Wang et al.
patent: 4510251 (1985-04-01), Kirkemo et al.
patent: 4585862 (1986-04-01), Wang et al.
patent: 4588697 (1986-05-01), Khanna et al.
patent: 4593089 (1986-06-01), Wang et al.
patent: 4681859 (1987-07-01), Kramer
Shipchandler, M. T., "4'-[Aminomethyl]fluorescein and its N-Alkyl Derivatives: Useful Reagents in Immunodiagnostic Techniques", Analytical Biochemistry, 162:89-101 (1987).
Miceli, Joseph N., et al., "An Improved Method for the Quantitation of Phencyclide (PCP) in Biological Samples utilizing Nitrogen-Detection Gas Chromatography", Journal of Analytical Toxicology, 5:29-32, Jan./Feb., 1981.
Lewellen, Larry J., et al., "Nitrogen-Sensitive Gas Chromatographic Detection and Quantitation of Nanogram Levels of Phencyclidine in Whole Blood", Journal of Analytical Toxicology, 72-75, Mar./Apr. 1979.
Egen, N. B., et al., "Isolation of Monoclonal Antibodies to Phencyclidine from Ascites Fluid by Preparative Isoelectric Focusing in the RotoFor", Anal. Biochemistry 172, 488-494 (1988).
Tillotson, J. A., et al., "The Fluorometric Apoprotein Titration of Urinary Riboflavin", Anal. Biochemistry 107, 214-219 (1980).
Rhodes, M. B., et al., "The Flavoprotein-Apoprotein System of Egg White", J. Biol. Chemistry 234, No. 8, 2054-2060 (1959).
Nishikimi et al., "Flavin-Protein Interaction in Egg White Flavoprotein", J. Biochem. 73, 1233-1242 (1973).
Murthy, U. S., et al., "The Interaction of Riboflavin with a Protein Isolated from Hen's Egg White: A Spectrofluorimetric Study", Biochimica et Biophysica Acta. 434, 69-81 (1976).
Heveran, J. E., "Determination of Phencyclidine by Radioimmunoassay", J. Forensic Sciences, vol. 25, No. 1, pp. 79-87 (1980).
Laboratory Techniques in Biochemistry and Molecular Biology, vol. 4, Part I, Work eral, North-Holland Publishing Co. Inc., (1975) pp. 78-79.

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