Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1990-08-06
1992-06-23
Ford, John M.
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
544375, C07D40512, C07D40506
Patent
active
051244578
ABSTRACT:
The present invention is directed to a fluorescence polarization assay for phencyclidine and phencyclidine derivatives, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them. The tracers and the immunogens are made from substituted phencyclidine compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.
REFERENCES:
patent: 4196185 (1980-04-01), Focella et al.
patent: 4255329 (1981-03-01), Ullman
patent: 4281065 (1981-07-01), Lin et al.
patent: 4351760 (1982-09-01), Khanna et al.
patent: 4420568 (1983-12-01), Wang et al.
patent: 4446065 (1984-05-01), Lin et al.
patent: 4476228 (1984-10-01), Huckzermeier et al.
patent: 4476229 (1984-10-01), Fino et al.
patent: 4481136 (1984-11-01), Khanna et al.
patent: 4492762 (1985-01-01), Wang et al.
patent: 4510251 (1985-04-01), Kirkemo et al.
patent: 4585862 (1986-04-01), Wang et al.
patent: 4588697 (1986-05-01), Khanna et al.
patent: 4593089 (1986-06-01), Wang et al.
patent: 4681859 (1987-07-01), Kramer
Shipchandler, M. T., et al., "4'-[Aminomethyl]fluorescein and its N-Alkyl Derivatives: Useful Reagents in Immunodiagnostic Techniques". Analytical Biochemistry, 162:89-101 (1987).
Miceli, Joseph N., et al., "An Improved Method for the Quantitation of Phencyclide (PCP) in Biological Samples Utilizing Nitrogen-Detection Gas Chromatography", Journal of Analytical Toxicology, 5:29-32, Jan./Feb., 1981.
Lewellen, Larry J., et al., "Nitrogen-Sensitive Gas Chromatographic Detection and Quantitation of Nanogram Levels of Phencyclidine in Whole Blood", Journal of Analytical Toxicology, 72-75, Mar./Apr., 1979.
Egen, N. B. et al., "Isolation of Monoclonal Antibodies to Phencyclidine from Ascites Fluid by Preparative Isoelectric Focusing in the Rotofor, " Anal. Biochemistry 172, 488-494 (1988).
Tillotson, J. A., et al., "Fluorometric Apoprotein Titration of Urinary Riboflavin," Anal. Biol. Chemistry 107,214-219 (1980).
Rhodes, M. B., et al., "The Flavoprotein-Apopreotein System of Egg White," J. Biol. Chemistry 234, No. 8, 2054-2060 (1959).
Nishikimi et al., "Flavin-Protein Interaction in Egg White Flavoprotein," J. Biochem. 73, 1233-1242 (1973).
Murthy, U. S., et al., "The Interaction of Riboflavin with a Protein Isolated from Hen's Egg White: A Spectrofluorimetric Study." Biochimica et Biophysica Acta, 434, 69-81 (1976).
Heveran, J. E., "Determination of Phencyclidine by Radioimmunoassay," J. Forensic Sciences, vol. 25, No. 1, pp. 79-87 (1980).
Nam Daniel S.
Ungemach Frank S.
Abbott Laboratories
Breininger Thomas M.
Ford John M.
LandOfFree
Phencyclidine and phencyclidine metabolites assay, tracers, immu does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Phencyclidine and phencyclidine metabolites assay, tracers, immu, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Phencyclidine and phencyclidine metabolites assay, tracers, immu will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-934050