Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Signal modification or steric inhibition
Patent
1992-04-27
1995-04-18
Kim, Kay K. A.
Chemistry: analytical and immunological testing
Involving immune complex formed in liquid phase
Signal modification or steric inhibition
436536, 436548, 436816, 43524027, 5303889, 5303898, 4241411, G01N 33542, G01N 33577, C12N 520, C07K 1528
Patent
active
054078342
ABSTRACT:
The present invention is directed to a fluorescence polarization assay for phencyclidine and phencyclidine derivatives, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them, and a reagent kit containing them. The tracers and the immunogens are made from substituted phencyclidine compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.
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Dubler Robert E.
Frintner Mary P.
Grote Jonathan
Hadley Gregg A.
Hawksworth David J.
Abbott Laboratories
Kim Kay K. A.
Pope Lawrence S.
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