Phencyclidine and phencyclidine metabolites assay, tracers, immu

Chemistry: analytical and immunological testing – Involving immune complex formed in liquid phase – Signal modification or steric inhibition

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

436536, 436548, 436816, 43524027, 5303889, 5303898, 4241411, G01N 33542, G01N 33577, C12N 520, C07K 1528

Patent

active

054078342

ABSTRACT:
The present invention is directed to a fluorescence polarization assay for phencyclidine and phencyclidine derivatives, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for making them, and a reagent kit containing them. The tracers and the immunogens are made from substituted phencyclidine compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane polarized light that has been passed through a sample containing antiserum and tracer.

REFERENCES:
patent: 4196185 (1980-04-01), Focella et al.
patent: 4255329 (1981-03-01), Ullman
patent: 4281065 (1981-07-01), Lin et al.
patent: 4351760 (1982-09-01), Khanna et al.
patent: 4420568 (1983-12-01), Wang et al.
patent: 4446065 (1984-05-01), Lin et al.
patent: 4476228 (1984-10-01), Huckzermeir et al.
patent: 4476229 (1984-10-01), Fino et al.
patent: 4481136 (1984-11-01), Khanna et al.
patent: 4492762 (1985-01-01), Wang et al.
patent: 4510251 (1985-04-01), Kirkemo et al.
patent: 4585862 (1986-04-01), Wang et al.
patent: 4588697 (1986-05-01), Khanna et al.
patent: 4593089 (1986-06-01), Wang et al.
patent: 4681859 (1987-07-01), Kramer
patent: 4939264 (1990-07-01), Heiman et al.
patent: 5124457 (1992-06-01), Ungenach et al.
patent: 5221629 (1993-06-01), Ungemach et al.
S. Seaver (Aug., 1994) Genetic Engineering News, pp. 10 and 21.
Kaul et al (1980) Clinical Toxicology 16(1):7-15.
Owens et al (1986) Drug Methabolism and Disposition 14(1):52-58.
Shipchandler, M. T., "4'-[Aminomethyl]fluorescein and its N-Alkyl Derivatives: Useful Reagents in Immunodiagnostic Techniques", Analytical Biochemistry, 162:89-101(1987).
Miceli, Joseph N., et al., "An Improved Method for the Quantitation of Phencyclide (PCP) in Biological Samples Utilizing Nitrogen-Detection Gas Chromatography", Journal of Analytical Toxicology, 5:29-32, Jan./Feb., 1981.
Lewellen, Larry J., et al., "Nitrogen-Sensitive Gas Chromatographic Detection and Quantitation of Nanogram Levels of Phencyclidine in Whole Blood", Journal of Analytical Toxicology, 72-75, Mar./Apr. 1979.
Egen, N. B., et al., "Isolation of Monoclonal Antibodies to Phencyclidine from Ascites Fluid by Preparative Isoelectric Focusing in the RotoFor," Anal. Biochemistry 172, 488-494 (1988).
Tillotson, J. A., et al., "The Fluorometric Apoprotein Titration of Urinary Riboflavin," Anal. Biochemistry 107, 214-219 (1980).
Rhodes, M. B., et al., "The Flavoprotein-Apoprotein System of Egg White," J. Biol. Chemistry 234, No. 8, 2060 (1959).
Nishikimi et al., "Flavin-Protein Interaction in Egg White Flavoprotein," J. Biochem. 73, 1233-1242 (1973).
Murthy, U.S., et al., "The Interaction of Riboflavin with a Protein Isolated from Hen's Egg White: A Spectrofluorimetric Study," Biochemica et Biophysica Acta, 434, 69-81 (1976).
Heveran, J. E., "Determination of Phencyclidine by Radioimmunoassay, " J. Forensic Sciences, vol. 25, No. 1, pp. 79-87 (1980).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Phencyclidine and phencyclidine metabolites assay, tracers, immu does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Phencyclidine and phencyclidine metabolites assay, tracers, immu, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Phencyclidine and phencyclidine metabolites assay, tracers, immu will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-66442

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.