Phenalenimine Fluorescent dyes and their use in analytical metho

Details Phenalenimine Fluorescent dyes and their use in analytical metho Phenalenimine Fluorescent dyes and their use in analytical metho

1988-07-05

1991-10-08

Lacey, David L.

Chemistry: analytical and immunological testing

Biospecific ligand binding assay

436528, 436800, 436546, 436805, 435 4, 435 34, 564427, C12Q 102, G01N 3353

Patent

active

050554140

ABSTRACT:
Certain phenalenimine fluorescent compounds represented by the structure ##STR1## wherein R' and R" are independently hydrogen, alkyl, cycloalkyl, aryl or a heterocycle, or R' comprises the carbon and heteroatoms which forms a fused ring with the compound nucleus, are useful in biomedical and analytical determinations. These compounds can be used for staining cells, as well as for the determination of various analytes found in human or animal biological fluids. Such determination can be carried out in solution or by using dry analytical elements. The fluorescent compounds can be reacted with quinone nuclei to form reducible compounds which are also useful in analytical methods. In addition, the compounds can be incorporated into what are known as "loadable" latex particles to form detectable labels and biological reagents.

REFERENCES:
patent: 4564698 (1986-01-01), Wieringa et al.
patent: 4592361 (1986-06-01), Parker et al.
patent: 4803161 (1989-02-01), Babb et al.
U.S.S.N. 824,757 (filed 1/31/86 by Babb) copending and commonly assigned.
U.S.S.N. 824,766 (filed 1/31/86 by Belly et al.) copending and commonly assigned.
Franz et al., Tetrahedron, 34, pp. 2147-2151 (1978).
Solodar et al., Zh. Orgn. Khim., 16(5), pp. 1062-1064, 1980 (English language translation).

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