Phase supports for the partition chromatography of macromolecule

Details Phase supports for the partition chromatography of macromolecule Phase supports for the partition chromatography of macromolecule

1987-07-20

1988-07-12

Therkorn, Ernest G.

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210656, 435182, 435803, 530413, B01D 1508

Patent

active

047568348

ABSTRACT:
Phase supports are disclosured for the partition chromatography of macromolecules. The phase supports are composed of non-adsorptive base support particles which are insoluble in the phase system and which have an average particle size within the range from 7 to 2000 .mu.m. The surface of the support particles is coated with a material which is insoluble in the phase system, adheres firmly to the particles and has an affinity for one of the phases of the phase system for the partition chromatography. Also disclosed is a process for the preparation of the phase supports and their use for separating, by partition chromatography, macromolecules, biopolymers, subcellular units and whole cells, particularly in an aqueous polyethylene glycol/dextran two-phase system.

REFERENCES:
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Snyder, Introduction to Liquid Chromatography, John Wiley & Sons, Inc., New York, 1979, pp. 272-278 and 532-533.

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