Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1986-12-19
1988-02-02
Therkorn, Ernest G.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
435 7, 210635, 424 85, 424105, A01N 102, A61K 3552, B01D 1508
Patent
active
047228873
ABSTRACT:
Male- and female-determining sperm are separated by polymeric phase partition chromatography, based on differential expression of a sulfoglycolipid. In a preferred embodiment, a mobile phase comprises a polymer derivatized with a sulfoglycolipid-binding protein, such as an antibody directed against the sulfoglycolipid. The sulfoglycolipid is preferably SGG.
REFERENCES:
patent: 4191749 (1980-03-01), Bryant
patent: 4448767 (1984-05-01), Bryant
Erickson and Glass, Functional Differences Between Sperm Bearing the X or Y Chromosome, Prospects for Sexing Mammalian Sperm, 1982, pp. 201-223.
Kaneko, "Separation of Human X and Y Bearing Sperm Using Percoll Density Gradient Centrifugation", Fertility and Sterility, vol. 40, No. 5, Nov. 1983, pp. 661-665.
Gledhill, "Control of Mammalian Sex Ratio by Sexing Sperm", Fertility and Sterility, vol. 40, No. 5, Nov. 1983, pp. 572-574.
Albertson, "Separation of Particles and Macromolecules by Phase Separation Endeavour", vol. 1, No. 2, 1978, pp. 69-74.
Walter et al., "Surface Differences Between Erythrocytes from Arbitrarily Chosen (Presumably Hematologically Normal) Individuals Detected by Cell Partitioning", Biochemical and Biophysical Research Communications, vol. 120, No. 1, Apr. 1984, pp. 250-255.
Fisher, "Cell Separations and Subfractionations by Countercurrent Distribution in Two Polymer Phase Systems Depend on Non Equilibrium Conditions", Biochem. and Biophys. 801 (1984), pp. 111-116.
Walter et al., "Detection of Surface Differences Between Two Closely Related Cell Populations by Partitioning", Biochem. and Biophys. 855 (1986) pp. 8-15.
Sharpe et al., "Studies of the Growth of Human Bone Derived Cells in Culture Using Aqueous Two-Phase Partition", Bioscience Reports vol. 4 (1984) pp. 415-419.
Sharpe, "Use of Aqueous Two-Phase Partition to Detect Cell Surface Changes During Growth of Dictoystelium Discoideum", J. Cell Science, vol. 75, 1985, pp. 339-346.
Sharpe, "Synthesis and Application of a Polyethylene Glycol-Antibody Affinity Ligand for Cell Separations in Aqueous Polymer Two-Phase Systems", Analytical Biochemistry, vol. 154, pp. 110-117 (1986).
Hammerstedt, "Potentials of Physical and Biochemical Separation Methods", 1982, pp. 169-175.
Hoppe, "Reacting Mouse Sperm with Monoclonal H-Y Antibodies Does Not Influence Sex Ratio of Eggs Fertilized in Vitro", Journal of Reproductive Immunology 6, 1984, pp. 1-9.
Schmell, "Identification of Mammalian Sperm Surface Antigens", Journal of Reproductive Immunology, vol. 4, 1982, pp. 91-106.
Lingwood, "Tissue Distribution of Sulfolipids in the Rat", Can. J. Biochem. vol. 59, 1981, pp. 556-563.
Lingwood, "The Preparation of Rabbit Antiserum Specific for Mammalian Testicular Sulfogalactoglycerolipid", Journal of Immunology, vol. 124, No. 2, Feb. 1980, pp. 769-774.
Lingwood, "Localization of Sulfatoxygalactosylacylalkylglycerol", Journal of Cell Biology, vol. 89, 1981, pp. 621-630.
Smith, "Rapid Communication", Journal of Experimental Zoology, vol. 225, 1983, pp. 157-160.
Myles, "Surface Domains of the Guinea Pig Sperm Defined with Monoclonal Antibodies", vol. 23 (1981), pp. 433-438.
Peterson, "Rapid Communication", Journal of Experimental Zoology, vol. 223 (1982), pp. 79-81.
Schmell, "Identification of Mammalian Sperm Surface Antigens", Fertility and Sterility, vol. 37, No. 2, Feb. 1982, pp. 249-257.
Shirley, "Enrichment of Sulfogalactosylalkylacylglycerol", Can. J. Biochem., vol. 58, 1980, pp. 1230-1239.
Nicholson, "The Sperm Membrane", Prospects for Sexing Mammalian Sperm, 1982, pp. 42-47.
Reproduction Research Information Services, "Bibliography on Separation of X and Y Spermatozoa", vol. 32, No. 4, pp. 326-328.
Fabricant Jill D.
Willett James D.
Biosyne Corporation
Cooper Iver P.
Therkorn Ernest G.
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