Electricity: measuring and testing – Particle precession resonance – Using a nuclear resonance spectrometer system
Patent
1987-10-20
1988-08-23
Tokar, Michael J.
Electricity: measuring and testing
Particle precession resonance
Using a nuclear resonance spectrometer system
324309, G01R 3320
Patent
active
047663774
ABSTRACT:
There is disclosed a method of precisely correcting the phase of a two-dimensional NMR spectrum along the two axes. First, a first phase train is applied to a sample. After an evolution period of t.sub.1, a second pulse train is applied. Then, the free induction decay signal emanating from the sample is detected during a detection period of t.sub.2, using a quadrature detection system. This process is repeated with different values of t.sub.1. Thus, a complex NMR spectrum S.sub.1 (t.sub.1, t.sub.2) is obtained. Subsequently, the sample is excited with a pulse sequence which is similar to the first-mentioned pulse sequence except that the second pulse is shifted in phase by 90.degree.
(n is a natural number) with respect to the second pulse of the first-mentioned pulse sequence. The resulting free induction decay signal is detected. This process is repeated with the same number of different values of t.sub.1 as the values of t.sub.1 used in the first set of measurements. Thus, a complex NMR spectrum S.sub.2 (t.sub.1, t.sub.2) is derived. The spectra S.sub.1 (t.sub.1, t.sub. 2) and S.sub.2 (t.sub.1, t.sub.2) are subjected to complex Fourier transformation with respect to the axis t.sub.2. Then, the imaginary part of S.sub.1 (t.sub.1, F.sub.2) and the real part of S.sub.2 (t.sub.1, F.sub.2) are interchanged to give rise to Fourier-transformed spectra S.sub.1 '(t.sub.1, F.sub.2) and S.sub.2 '(t.sub.1, F.sub.2), which are then subjected to complex Fourier transformation with respect to the axis t.sub.1. The phase is corrected along one of the axes. The imaginary part of S.sub.1 '(F.sub.1, F.sub.2) and the real part of S.sub.2 (F.sub.1, F.sub.2) are interchanged. Then, the phase is corrected along the other axis.
REFERENCES:
patent: 3873908 (1975-03-01), Young
patent: 4706027 (1987-11-01), Hughes
patent: 4713614 (1987-12-01), Hoshino
patent: 4720679 (1988-01-01), Patrick et al.
D. Marion and K. Wuthrich, "Application of Phase Sensitive Two-Dimensional Correlated Spectroscopy (COSY) For Measurements of .sup.1 H--.sup.1 H Spin-Spin Coupling Constants in Proteins", Received May 18, 1983, Biochemical and Biophysical Research Communications, vol. 113, No. 3, 1983, Jun. 29, 1983, pp. 967-974.
D. J. States, R. A. Haberkorn and D. J. Ruben, "A Two-Dimensional Nuclear Overhauser Experiment with Pure Absorption Phase in Four Quadrants", Received Dec. 29, 1981, Journal of Magnetic Resonance 48, 286-292 (1982).
Ohuchi Muneki
Uchida Soukichi
Jeol Ltd.
Tokar Michael J.
LandOfFree
Phase correction method in two-dimensional NMR spectroscopy does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Phase correction method in two-dimensional NMR spectroscopy, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Phase correction method in two-dimensional NMR spectroscopy will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-837625