Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Separation of micro-organism from culture media
Reexamination Certificate
2003-06-24
2009-11-03
Lankford, L Blaine (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Separation of micro-organism from culture media
C435S243000, C435S244000, C435S252100
Reexamination Certificate
active
07611885
ABSTRACT:
The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011minicells.
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Office Action dated Dec. 24, 2008 for U.S. Appl. No. 11/691,698.
Brahmbhatt Himanshu
MacDiarmid Jennifer
Bent Stephen A.
Engeneic Molecular Delivery Pty, Ltd.
Foley & Lardner LLP
Lankford L Blaine
McCaslin R. Brian
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