Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2011-04-19
2011-04-19
Kim, Alexander D (Department: 1656)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S194000, C435S252300
Reexamination Certificate
active
07928206
ABSTRACT:
This invention relates to novel plant thymidine kinases and their use in gene therapy. More specifically the invention provides novel thymidine kinases derived from tomato.In further aspects the invention provides novel polynucleotides encoding the tomato thymidine kinase of mutant thereof, vector constructs comprising the polynucleotide, host cells carrying the polynucleotide or vector, methods of sensitising cells to prodrugs, methods of inhibiting pathogenic agents in warm-blooded animals, methods for biocontrol of plants, methods of synthesizing monophosphates and pharmaceutical compositions comprising the plant thymidine kinases of the invention.In a preferred embodiment the invention provides a unique combination of a plant thymidine kinase and the nucleoside analog nucleoside analog AZT (3′-azido-3′-deoxythymidine) to treat abnormal cell growth.
REFERENCES:
patent: 5877010 (1999-03-01), Loeb et al.
patent: 6197743 (2001-03-01), Faller
patent: WO 99/19466 (1999-04-01), None
patent: WO 99/67372 (1999-12-01), None
patent: WO 01/79502 (2001-10-01), None
Munir et al (1993) Proc. Natl. Acad. Sci. USA, vol. 90, p. 4012-4016.
Boom et al (1990, Journal of Clinical Microbiology, vol. 28, p. 495-503).
Tris MSDS (Feb. 2002).
EDTA MSDS (2000).
Munir et al. (1992) The Journal of Biological Chemistry, vol. 267, p. 6584-6589.
Sanger et al., (1977) DNA sequencing with chain-terminating inhibitors, vol. 74, p. 5463-5467.
Oliver et al. (1997) The Journal of Biological Chemistry, vol. 272, pp. 10624-10630.
Zhang et al., A strategy for rapid cDNA cloning from double-stranded RNA templates isolated from plants infected with RNA viruses by using Taq DNA polymerase., Journal of Virological Methods, 2000, vol. 84, pp. 59-63.
Strauss et al., Plasmid Expressing theHerpes simplexVirus Thymidine Kinase Gene in Mammalian and Bacterial Cells., Mol Gen Genet, 1983, vol. 191, pp. 154-157.
Wacey et al., Disentangling the perturbational effects of amino acid substitutions in the DNA-binding domain of p53., Hum Genet, 1999, vol. 104, pp. 15-22.
Kizana et al., Therapeutic Prospects of Cardiac Gene Transfer, Heart, Lung and Circulation, 2007, vol. 16, pp. 180-184.
Berenstein, Dvora et al., Valine, Not Methionine, Is Amino Acid 106 in Human Cytosolic Thymidine Kinase (TK1), article, 2000, pp. 32187-32192, vol. 275, No. 41, The Journal of Biological Chemistry.
Kokoris, Mark et al., “Characterization ofHerpes simplexvirus type 1 thymidine kinase mutant engineered for improved ganciclovir or acyclovir activity”, article, Sep. 2002, pp. 2267-2272, vol. 11, No. 9, Protein Science: A Publication of the Protein Society, United States of America.
Munir , K.M. et al., “Thymidine kinase mutants obtained by random sequence selection.”, abstract only, May 1, 1993, 1 page, vol. 11, No. 9, Proceedings of the National Academy of Sciences of the United States of America.
NCBI, Sequence printout for AAF1307, putative thymidine kinase, GI:6466962, PLN Jan. 24, 2001, based on Lin, et al., “Arabidopsis thalianachromosome III P1 MLP3 genomic sequence” [data base accessed Oct. 29, 2004].
NCBI, Sequence printout for AF066050,Oryza sativathymidine kinases, GI:3411151, PLN Jun. 17, 1999, based on Ullah, et al., “A gene for Thymidine Kinase in Plants”, Plant Physiol., 119(4):1567, (1999). [data base accessed Apr. 20, 2004].
NCBI, Sequence printout for BAB09824, Contains similarity to thymidine kinase-gene, GI:9759365, PLN Dec. 27, 2000, based on Kotani et al., “Structural analysis ofArabidopsis thalianachromosome”, DNA Res. , 4,(4):291-300 (1997) [data base accessed Oct. 29, 2004].
Pantuck, Allan J., et al., “Optimizing Prostate Cancer Suicide Gene Therapy UsingHerpes simplexVirus Thymidine Kinase Active Site Variants”, article, May 1, 2002, pp. 777-789, vol. 13, Human Gene Therapy.
Ullah, Md. Hemayet et al., “A Gene for Thymidine Kinase in Plants”, abstract only, 1999, pp. 1567-1568; 1-2, vol. 119, American Society of Plant Physiologists.
Sequence, BE463259, GenBank gi 9509032, NCBI Sequence Viewer Aug. 15, 2005.
Knecht, et al., “Deoxyribonucleoside Kinase Belonging to the Thymidine Kinases 2 (TK2)—like Group Vary significantly in Substrate Specificity, Kinetics and Feed-back Regulation”,J. Mol. Biol., vol. 315, pp. 529-540, 2002.
Knecht, et al., “Identification of Residues Involved in the Specificity and Regulation of the Highly Efficient Multisubstrate Deoxyribonucleoside Kinase fromDrosophila melanogaster”, J. Mol. Biol., vol. 301, pp. 827-837, 2000.
Munch-Petersen, et al., “Functional Expression of a Multisubstrate Deoxyribonucleoside Kinase fromDrosophila melanogasterand Its C-terminal Deletion Mutants”,The Journal of Biological Chemistry, vol. 275, No. 9, pp. 6673-6679, Mar. 3, 2000.
Piskur, Jure (Supervisor), “In-Vitro Study of Novel Deoxyribonucleoside Kinases for Gene Therapy”, Department of Microbiology, Technical University of Denmark, Cyrille Le Breton Mar.-Aug. 2002.
Sequence, AU068889, GenBank gi 5003740, NCBI Sequence Viewer [Dec. 11, 2004].
Sequence, AW755132, GenBank gi 7676852, NCBI Sequence Viewer [Dec. 11, 2004].
Sequence, BE563259, GenBank gi 9807071, NCBI Sequence Viewer [Dec. 11, 2004].
Sequence, BG129197, GenBank gi 12629385, NCBI Sequence Viewer [Dec. 11, 2004].
Sequence, D24903, GenBank gi 428749, NCBI Sequence Viewer [Dec. 11, 2004].
NCBI, GenBank AF514775, “Lycopersicon esculentumTK1-like deoxyribonucleoside kinase mRNA, complete cds”, submitted to GenBank by Sandrini, Knecht and Piskur on May 22, 2002, last modified, Jan. 1, 2004.
Knecht Wolfgang
Munch-Petersen Birgitte
Piskur Jure
Cooper Iver P.
Kim Alexander D
LandOfFree
Pharmaceutical composition comprising a thymidine kinase... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Pharmaceutical composition comprising a thymidine kinase..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Pharmaceutical composition comprising a thymidine kinase... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2636976