Phagemids and process of preparation

Details Phagemids and process of preparation Phagemids and process of preparation

1997-06-23

1999-07-20

Degen, Nancy

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 692, 435 697, 4352351, 4352573, C12P 2102, C12N 121, C12N 701

Patent

active

059255595

ABSTRACT:
The invention concerns a collection of phagemids made up of a promotor, a gene of a fusion between a ligand protein, wherein the ligand protein in its processed state would be of from 40 to 60 amino acid residues, and a bacteriophage protein, at least one transcription terminator sequence, a replication origin derived from a single strand bacteriophage, a plasmid replication origin and facultatively at least one selection marker. Further, the invention concerns a collection of Escherichia coli clones or cells representing the collection of phagemids, a process for isolating phagemid particles with strong binding characteristics for a defined target molecule with the use of collections according to the invention and phagemid particles obtained according to the process.

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