Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-07-19
1998-10-20
Chambers, Jasemine C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 914, 435 6, 4353201, 435375, 4351723, 935 22, 935 23, 514 44, C12P 1934, C12N 1513, C12N 1564, C12N 1579
Patent
active
058245200
ABSTRACT:
One aspect of the invention relates to a phage-display library that expresses single-chain recombinant binding proteins. Inserts in the library comprise immunoglobulin heavy chain framework regions flanking highly divergent, synthetically produced hypervariable regions. A second aspect of the invention relates to the use of single-chain recombinant binding proteins to inhibit the activity of an intracellular constituent. In the exemplary case presented, the activity of intracellular glucose-6-phosphate dehydrogenase was inhibited by intracellular expression of a cloned single-chain recombinant binding protein.
REFERENCES:
patent: 5702892 (1997-12-01), Mulligan-Kehoe
Current Protocols in Molecular Biology, "Unit 3.17: Constructing recombinant DNA molecules by the polymerase chain reaction", Ausubel et al., eds. John Wiley & Sons, Inc., 1993.
Methods of Enzymatic Analysis, vol. 1, Bergmeyer, ed., Academic Press, Inc., New York, NY, pp. 458-459, 1974.
Molecular Cloning: A Laboratory Manual, Sambrook et al., eds., Cold Pring Harbor Lab. Press, Cold Spring Harbor, NY, pp. 4.1-4.54, 1989.
Orkin et al., "Report and recommendations of the panel to assess the NIH investment in research on gene therapy", issued by the U.S. National Institutes of Health, Dec. 1995.
Marasco, "Intrabodies: turning the humoral immune system outside in for intracellular immunization", Gene Therapy 4: 11-15, Jan. 1997.
Biocca et al, "Intracellular immunization with cytosolic recombinant antibodies", Bio/Technol. 12: 396-399, Apr. 1994.
Davies et al., "Antibody VH domains as small recognition units", Bio/Technol. 13: 475-479, May 4, 1995.
Carlson, J.R. (1988) A new means of inducibly inactivating a cellular protein, Molecular and Cellular Biology, 8(6):2638-2646.
Little et al, (1994) Surface display of antibodies, Biotechnology Advances. 12:539-555.
Barbas, C., et al. (1993) Direct selection of antibodies that coordinate metals from semisynthetic combinatorial libraries. Proc. Natl. Acad. Sci. 90:6385-6389.
Barbas, C., et al. (1992) Semisynthetic combinatorial anitbody libraries: a chemical solution to the diversity problem. Proc. Natl. Acad. Sci. 89:4457-4461.
Bradford, M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of proteing utilizing the principle of protein-dye binding, Analytical Biochemistry 72:248-254.
Deng, S., et al. (1993) Simultaneous randomization of antibody CDRs by a synthetic ligase chain reaction strategy, Nucleic Acids Research 21(18):4418-4419.
Holliger, P., et al. (1993) "Diabodies": small bivalent and bispecific antibody fragments. Proc. Natl. Acad. Sci. 90:6444-6448.
Hozumi, N., et al. (1993) Recombinant antibody technology: its advent and advances. Cancer Investigation 11(6):714-723.
Huse, W., et al. (1989) Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda, Science 246:1275-1281.
Kang, A., et al. (1991) Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces, Proc. Natl. Acad. Sci. 88:4363-4366.
Kohler, G., et al. 1975) Continuous cultures of fused cells secreting antibody of predefined specificity, Nature 256:495-497.
Kohler, G., et al. (1976) Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6:511-519.
Kozak, M. (1981) Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Research 9(20):5233-5252.
Mullinax, R., et al., (1990) Identification of human antibody fragment clones specific for tetanus toxoid in a bacteriophage . . . Proc. Natl. Acad. Sci. 87:8095-8099.
Oldenberg, K., et al., (1992) Peptide ligands for a sugar-binding protein isolated from a random peptide library. Proc. Natl. Acad. Sci. 89:5393-5397.
Posner, B., et al., (1993) A revised strategy for cloning antibody gene fragments in bacteria, Gene 128:111-117.
Sharon, J. (1990) Structural correlated of high antibody affinity: three engineered amino acid substitutions can increase the affinity of an anti-p-azophenylarsonate andtibody 200-fold. Proc. Natl. Acad. Sci. 87:4814-4817.
Chambers Jasemine C.
Priebe Scott D.
The United States of America as represented by the Department of
LandOfFree
Phage-display of immunoglobulin heavy chain libraries for identi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Phage-display of immunoglobulin heavy chain libraries for identi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Phage-display of immunoglobulin heavy chain libraries for identi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-243286