Phage-cosmid hybrid vector, open cos DNA fragments, their method

Details Phage-cosmid hybrid vector, open cos DNA fragments, their method Phage-cosmid hybrid vector, open cos DNA fragments, their method

1995-04-07

1997-12-09

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43525233, 4353201, 935 52, 935 55, 935 56, 935 58, 935 73, 536 241, 536 242, C12N 1500, C12N 120, C07H 2104

Patent

active

056959710

ABSTRACT:
A hybrid vector produced from a linearized cosmid and arms from .lambda. phage vector DNA has been developed. The hybrid vector can be used to generate fragments which are useful as vectors in a helper phage-mediated transformation system, permitting large fragments of foreign DNA to be introduced into a host on an industrial scale.

REFERENCES:
patent: 4680264 (1987-07-01), Puhler et al.
patent: 5075227 (1991-12-01), Hagen
Watson et al., "Molecular Biology Of the Gene", 1965, pp. 210-211.
Shizuya et al., "Cloning And Stable Maintenance of 300-Kilobase-Pair Fragments Of Human DNA in Escherichia coliUsing An F-Factor-Based Vector", Proc. Natl. Acad. Sci, USA, vol. 89, Sep. 1992, pp. 8794-8797.
Chauthaiwale et al., "Bacteriophage Lamboda As A Cloning Vector", Microbiological Reviews, Dec. 1992, pp. 577-591.
Zuber et al., "Tn5cos: a transposon for restriction mapping of large plasmids using phage lambda terminase", Gene 103: 69-72, 1991.
Sambrook et al., "Molecular Cloning: A Laboratory Manual", 2nd edition, Cold Spring Harbor Laboratory Press, pp. 3.4, 2.42-2.43, 8.37-8.38, 3.28-3.31, E.34-E.36, 6.32-6.35, 2.85-2.87, and 2.12, 1989.
Enquist et al., Meth. Enzymol., pp. 281-298, 1979.

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