Phage-associated lytic enzymes for treatment of Bacillus...

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases

Reexamination Certificate

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C424S094670, C435S212000, C435S018000, C435S069100, C435S320100, C435S325000, C435S252300, C536S023200, C530S350000

Reexamination Certificate

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10849948

ABSTRACT:
The present disclosure relates to methods, compositions and articles of manufacture useful for the treatment ofBacillus anthracisandB. cereusbacteria and spores, and related conditions. The disclosure further relates to methods and compositions for the identification of a phage associated lytic enzyme to rapidly and specifically detect and killBacillus anthracisand other bacteria. Related articles of manufacture, methods of degrading spores and methods of treatment of infections or bacteria populations of, or subjects exposed to or at risk for exposure to,Bacillus anthracisare also provided.

REFERENCES:
patent: 2002/0127215 (2002-09-01), Loomis et al.
patent: WO 03/010316 (2003-02-01), None
Branden et al., Introduction to Protein Structure, Garland Publishing Inc., New York, p. 247, 1991.
Witkowski et al., Biochemistry 38:11643-11650, 1999.
Seffernick et al., J. Bacteriol. 183(8):2405-2410, 2001.
Brown, Eric R. et al.; “Specific Identification ofBacillus Anthracisby Means of a Variant Bacteriophage”; United States Department of Health, Education and Welfare, Communicable Disease Center, Public Health Service, Chamblee, Georgia, pp. 34-39., 1954.
Nelson, Daniel et al.; “Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme”; Proc. Natl. Acad. Sci., 2001, vol. 98, No. 7, pp. 4107-4112.
Schuch, Raymond et al.; “A bacteriolytic agent that detects and killsBacillus anthracis;”Nature, vol. 418; Aug. 22, 2002; pp. 884-889.
Internet excerpt entitled “Bacteriophage;” New Horizons Diagnostics Inc.; obtained at the internet address: nhdiag.com/phage.shtml; Nov. 5, 2003; two pages.
Internet excerpt entitled “Bacteriophage;” New Horizons Diagnostics Inc.; obtained at the internet address: nhdiag.com/phage.shtml; Jan. 13, 2004; two pages.
Internet excerpt entitled “Bacillus anthracis(spore) Lateral Flow Screening Assay;” New Horizons Diagnostics Inc.; obtained at the internet address: nhdiag.com/anthrax.shtml; Jan. 13, 2004; two pages.

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