Phage antibodies

Details Phage antibodies Phage antibodies

2005-02-22

2005-02-22

Nickol, G (Department: 1642)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

C435S006120, C435S069600, C435S069900, C435S320100, C435S332000, C436S547000, C530S387100, C530S395000

Reexamination Certificate

active

06858384

ABSTRACT:
Peripheral blood leucocytes incubated with a semi-synthetic phage antibody library and fluorochrome-labeled CD3 and CD20 antibodies were used to isolate human single chain Fv antibodies specific for subsets of blood leucocytes by flow cytometry. Isolated phage antibodies showed exclusive binding to the subpopulation used for selection or displayed additional binding to a restricted population of other cells in the mixture. At least two phage antibodies appeared to display hithereto unknown staining patterns of B lineage cells. This approach provides a subtractive procedure to rapidly obtain human antibodies against known and novel surface antigens in their native configuration, expressed on phenotypically defined subpopulations of cells. Importantly, this approach does not depend on immunization procedures or the necessity to repeatedly construct phage antibody libraries.

REFERENCES:
patent: 6265150 (2001-07-01), Terstappen et al.
patent: 0610774 (1994-08-01), None
patent: WO 9426787 (1994-11-01), None
de Kruif (PNAS USA Apr. 1995;92:3938-3942).*
Winter et al (Annu. Rev. Immunol. 1994;12:433-455).

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