pH and pCO.sub.2 sensing by luminescent lifetimes and energy tra

Details pH and pCO.sub.2 sensing by luminescent lifetimes and energy tra pH and pCO.sub.2 sensing by luminescent lifetimes and energy tra

1995-03-14

1997-07-15

Snay, Jeffrey

Chemistry: analytical and immunological testing

Blood gas

436133, 436163, 436172, G01N 2164

Patent

active

056482698

ABSTRACT:
A method for determining pH or pCO.sub.2 using luminescent lifetimes and energy transfer in which an energy transfer donor-acceptor pair is exposed to a sample to be analyzed, the donor of the donor-acceptor pair being photoluminescent and the acceptor of the donor-acceptor pair being sensitive to the pH or pCO.sub.2 of the sample. One or both of the donor-acceptor pair may be bound to a carrier. The sample is irradiated and the resultant emission detected. By measuring the apparent luminescent lifetime, the pH or pCO.sub.2 of the sample can be determined.

REFERENCES:
patent: 4854321 (1989-08-01), Boiarski
patent: 5037615 (1991-08-01), Kane
patent: 5127405 (1992-07-01), Alcala et al.
"Applications of Fluorescence in the Biomedical Sciences" Lakowicz, J.R., Alan R. Liss, Inc., pp. 29-67 (1986).
Jordan, D.M. et al. "Physiological pH Fiber-Optic Chemical Sensor Based on Energy Transfer", Analytical Chemistry, vol. 59, pp. 437-439 (1987).
Lakowicz `Principles of Fluorescence Spectrocopy` Plenum Press, 1983.

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