Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2006-03-28
2006-03-28
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C800S279000, C435S320100, C435S418000, C536S023400
Reexamination Certificate
active
07019197
ABSTRACT:
Disclosed are polynucleotides encoding a pesticidal fusion polypeptide comprising (i) a toxin domain; and (ii) a heterologous binding domain capable of binding non-specifically to a cell membrane without disrupting that membrane. Preferably the toxin domain is derived from aBacillus thuringiensiscry toxin (e.g. CryIA (b) or (c)) and the binding domain is derived from a lectin (e.g. ricin toxin B chain). The use of such fusions may help to inhibit the acquisition of resistance in a pest population treated with the polypeptide. A further aspect of the invention is a method of assessing the toxicity of a polypeptide to a pest species by expressing a nucleic acid encoding said polypeptide in a host cell from that species, observing the viability of the cell and correlating the results of the observation with the toxicity of the polypeptide, wherein the viability is determined by assessing esterase activity or membrane.
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Christou Paul
Mehlo Luke
Dann Dorfman Herrell & Skillman P.C.
Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung
Kubelik Anne
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