Peroxidase colloidal gold oxidase biosensors for mediatorless gl

Chemistry: electrical and wave energy – Apparatus – Electrolytic

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

204415, 435817, G01N 2726

Patent

active

052250644

ABSTRACT:
Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The biodetectors may be employed for mediatorless detection of a wide variety of analytes depending on the oxidase employed.

REFERENCES:
patent: 4340448 (1982-07-01), Schiller et al.
patent: 4820399 (1989-04-01), Senda et al.
patent: 4970145 (1990-11-01), Bennetto et al.
patent: 5082550 (1992-01-01), Rishpon et al.
patent: 5082786 (1992-01-01), Nakamoto
Crumbliss, A. L. et al., "Catalytic and Electroactivity of Irreversibly Adsorbed Enzymes at Gold Electrode Surfaces," Symposium on Protein Electrochemistry: Abstract for the ACS Southeast Regional Meeting (SERM) Oct., 1989, published in USA.
Fair, B. D., and Jamieson, A. M., "Studies of Protein Adsorption on Polystyrene Latex Surfaces," J. Colloid Interface Sci., 77(2):525-534, 1980.
Frew, J. E. et al., "A Method for Estimation of Hydrogen Peroxide Based on Mediated Electron Transfer Reactions of Peroxidases at Electrodes," Electroanal. Chem., 201:1, 1986.
Govindaraju, K. et al., "Active Site Chemistry of Lysyl Oxidase," J. Inorg. Biochem., 29:111, 1987.
Gregg, B. A., and Heller, A., "Cross-Linked Redox Gels Containing Glucose Oxidase for Amperometric Biosensor Applications," Anal. Chem., 62:258-263, 1990.
Guo, L-H et al., "Direct Electrochemistry of Proteins and Enzymes," Adv. Inorg. Chem., 36:341-375, 1991.
Guo, L-H et al., "Direct Voltammetry of the Chromatium vinosum Enzyme, Sulfide: Cytochrome c Oxidoreductase (Flavocytochrome c552)," J. Biol. Chem., 265(4):1958-1963, 1990.
Guo, L-H et al., "Direct Un-Mediated Electrochemistry of the Enzyme p-Cresolmethylhydroxylase," J. Electroanal. Chem., 266:379-396, 1989.
Hagen, W. R., "Direct Electron Transfer of Redox Proteins at the Bare glassy Carbon Electrode," J. Biochem., 182:523-530, 1989.
Albery et al., "Inhibited Enzyme Electrodes. Part 3. A Sensor for Low Levels of H.sub.2 S and HCN," Biosensors & Bioelectronics, 5:397-413, 1990.
Armstrong, Fraser A., "Voltammetry of Metal Centres in Proteins," Perspectives on Bioinorganic Chemistry, 1:141-165, 1991.
Armstrong, F. A., and Lannon, A. M., "Fast Interfacial Electron Transfer between Cytochrome c Peroxidase and Graphite Electrodes Promoted by Aminoglycosides: Novel Electroenzymic Catalysis of H.sub.2 O.sub.2 Reduction, " J. Am. Chem. Soc., 109:7211-7212, 1987.
Bowden, E. F., and Hawkridge, F. M., "Interfacial Electrochemistry of Cytochrome c At Tin Oxide, Indium Oxide, Gold, and Platinum Electrodes," J. Electroanal. Chem., 161:355-376, 1984.
Cass, A. E. G., ed., Biosensors: A Practical Approach, Oxford University Press, Oxford/New York, 1990.
Crumbliss, A. L. et al., "Amperometric Glucose Sensor Fabricated from Glucose Oxidase and a Mediator Co-Immobilized on a Colloidal Gold Hydrogel Electrode," Abstract for the ACS North Carolina Divisional Meeting, University of North Carolina at Chapel Hill, Sep. 7-9, 1989, published in USA.
Crumbliss, A. L. et al., "The Use of Inorganic Materials to Control or Maintain Immobilized Enzyme Activity," Symposium on "opportunities for inorganic chemistry in biotechnology," Abstract for the ACS National Meeting in Boston, Apr. 23, 1990, published in USA.
Crumbliss, A. L. et al., "The Influence of Colloidal Gold Surfaces on Enzyme Activity," Abstract for the ACS North Carolina Divisional Meeting, Sep., 1988, published in USA.
Hale, P. D., et al., "Amperometric Glucose Biosensors Based on Redox Polymer-Mediated Electron Transfer," Anal. Chem., 63:677-682, 1991.
Henkens, R. W. et al., "Bioactive Electrodes Using Metallo-Proteins Attached to Colloidal Gold," Rec. Trav. Chem. Pays Bas., 106:6-7, 1987.
Henkens, R. W. et al., "Biosensor Electrodes Using Colloidal Gold Supported Oxidase Enzymes," J. Inorg. Biochem., 43:120, 1991.
Henkens, R. W., and O'Daly, J. P., "Multi-Analyte Enzyme Electrodes for Environmental Monitoring," Abstract for the Proceedings of 5th International Biotechnology Conference in Copenhagen, Jul. 8-13, 1990.
Jonsson, G., and Gorton, L., "An Electrochemical Sensor for Hydrogen Peroxide Based on Peroxidase Adsorbed on a Spectrographic Graphite Electrode," Electroanalysis, 1:465-468, 1989.
Kinoshita, Kim, "Carbon: Electrochemical and Physicochemical Properties," John Wiley & Sons, New York, 1988.
Paddock, R. M., and Bowden, E. F., "Electrocatalytic Reduction of Hydrogen Peroxide via Direct Electron Transfer from Pyrolytic Graphite Electrodes to Irreversibly Adsorbed Cytochrome c Peroxidase," J. Electroanal. Chem., 260:487-494, 1989.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Peroxidase colloidal gold oxidase biosensors for mediatorless gl does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Peroxidase colloidal gold oxidase biosensors for mediatorless gl, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Peroxidase colloidal gold oxidase biosensors for mediatorless gl will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1687363

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.