Peroxidase catalyzed enzyme assay by sample PRG-treatment

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol

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Details

435 12, 435 28, 436 13, 436 71, C12Q 160, C12Q 158, C12Q 128, G01N 3392

Patent

active

052468364

ABSTRACT:
A method for determination of an analyte in a sample containing reducing substances is disclosed. After the decomposition of the reducing substances by reaction with hydrogen peroxide formed by enzymatic Redox reaction using a component in the sample which does not participate in another enzymatic Redox reaction using the analyte, the remaining hydrogen peroxide is decomposed, and then the analyte is subjected to enzymatic Redox reaction to form hydrogen peroxide which is then determined by a known method.

REFERENCES:
patent: 4416982 (1983-11-01), Tsuda
patent: 4636464 (1987-01-01), Nakanishi
patent: 4681841 (1987-07-01), Matsumoto
patent: 4820416 (1989-04-01), Chang
patent: 4954451 (1990-09-01), Albarella

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