Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an...
Patent
1993-06-03
1998-06-23
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an...
435395, 435403, 436524, 436527, 502407, 530811, C12N 1114, C12N 500, G01N 33552, B01J 2010
Patent
active
057704163
ABSTRACT:
Permeable substantially spherical hollow particles of 1-5000 .mu.m in size are formed having an outer shell of a mechanically rigid porous material. Pores of the shell are through-going pores that connect the inside of the particles to surroundings and allow chemical species to traverse the outer shell. To prepare the particles, impermeable hollow particles are treated with an acid or base under reflux conditions to form pores. The outer shell is formed of anhydrous forms of silicon dioxide, metal silicates, metal borosilicates, metal oxides or boric oxide. Metals and metal alloys are not used in forming the outer shell. Particles containing a polymer are formed by immersing the permeable hollow particles in a solution of components that polymerize to form the polymer, allowing the solution to partially fill the particles via the pores and polymerizing the components. The permeable particles are useful as carriers in High Performance Liquid Chromatography, gel filtration, ion exchange, affinity chromatography, immunochemical procedures, solid-phase peptide or protein synthesis, immobilization of catalysts such as enzymes and noble metals and immobilization of cells or virus particles. The particles may also be used as filtration aids, adsorbents or absorbents.
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"Fillite" by Fillite (Roncorn) Ltd., Roncorn, Cheshire, U.K.
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Barany et al., Int. J. Peptide Proteins Res. 30 (1987) pp. 705-739.
B.o slashed.g-Hansen Thorkild Christian
Lihme Allan Otto Fog
Nielsen Claus Schafer
Naff David M.
Upfront Chromatography A/S
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