Periplasmic protein recovery

Details Periplasmic protein recovery Periplasmic protein recovery

1984-10-05

1987-07-14

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 435317, 435259, 435803, 935 48, C12P 2100, C12N 1500, C12N 106

Patent

active

046802622

ABSTRACT:
Periplasmic proteins are recovered from transformed gram negative bacteria by a process comprising freezing and thawing the cells. Advantages are obtained by culturing the cells in phosphate-limiting media and by killing the cells prior to separation of periplasmic proteins.

REFERENCES:
patent: 4275166 (1981-06-01), McCollough et al.
patent: 4497730 (1985-02-01), Ames et al.
Davis, B. D. and R. Dulbecco "Sterilization and Disinfection" In: Davis, B. D. et al. Microbiology, 3rd ed, (New York, Harpes and Row, 1980) pp. 1264-1274.
Bungay, H. R. et al., "Biochemical Engineering" In: Perry, R. H., et al., Perry's Chemical Engineers Handbook, 6th ed. (New York, McGraw-Hill) pp. 27-1-27-19.
McGregor, W. C. "Large-Scale Isolation and Purification of Proteins from Recombinant E. Coli" In: Venkatasubramanian, K. et al., Biochemical Engineering III, (New York, New York Academy of Sciences, 1983) pp. 231-237.
Inouye et al., "J. Bact." 149(2):434-439 (Feb. 1982).
Picken et al., "Infection and Immunity" 42(1):269-275 (Oct. 1983).
Michaelis et al., "J. Bact." 154(1):366-374 (Apr. 1983).
Miyake et al., "J. Biochem." 97:1429-1436 (1985).
Saier, M. H. "The Bacteria" VII (4) 218-222.
Atlan et al., "Appl. Microb. Biotechnol." 19:5-12 (1984).
Wanner, B. L. "J. Mol. Biol." 166:283-308 (1983).
Calcott et al., "Can. J. Microbiol." 30:339-344 (1984).
MacLeod et al., "Symp. Soc. Gen. Microbiol." 26:81-109 (1976).
Neu et al., "J. Biol. Chem." 240(9):3685-3692 (1965).

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