Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1987-08-14
1990-08-07
Mays, Thomas
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4351723, 43525233, 4352528, 435849, 935 14, 935 29, 935 48, 935 73, C12N 1500, C12N 120
Patent
active
049467832
ABSTRACT:
This invention features a method of isolating a mutant strain of Escherichia coli, having a defective periplasmic protease, the method comprising the steps of: mutagenizing an E. coli cell, wherein the cell comprises: (a) an inner and an outer membrane, (b) a periplasmic space between the membranes, (c) a protein which in a first state is mobile, being able to move through the outer membrane and enter medium surrounding the cells, the protein in the first state being detectable in the medium, and in a second state is not mobile, remaining inside the cell, and (d) a periplasmic protease which converts the protein from the second state to the first state in the cell, and selecting a mutant cell which produces a reduced level of the detectable protein in the medium compared to the E. coli cell, wherein the mutant cell comprises the defective periplasmic protease.
This invention also features mutant strains of E. coli having a defective periplasmic protease.
REFERENCES:
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Ichihara et al., "Protease IV, A Cytoplasmic Membrane Protein of Escherichia coli, Has Signal Peptide Peptidase Activity", The Journal of Biological Chemistry, vol. 259, No. 15, Issue of Aug. 10, pp. 9853-9857, 1984.
Suzuki, et al., "Characterization of the sppA Gene Coding for Protease IV, A Signal Peptide Peptidase of Escherichia coli", Journal of Bacteriology, Jun. 1987, pp. 2523-2528, vol. 169, no. 6.
Beckwith Jonathan R.
Strauch Kathryn L.
Mays Thomas
President and Fellows of Harvard College
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