Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;...
Patent
1999-02-24
2000-01-25
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
424422, 424 941, 435176, 435177, 435180, 435181, 436518, 436524, 436531, 436532, 530402, 530810, 530811, 530815, 530816, C12N 1100, G01N 33543, C07K 100, A61K 3843
Patent
active
060177411
ABSTRACT:
Methods are provided for forming a coating of an immobilized biomolecule on a surface of a medical device to impart improved biocompatibility for contacting tissue and bodily fluids. A 2-aminoalcohol moiety of a biomolecule is oxidized with a periodate to an aldehyde moiety which is reacted with an amine moiety on the surface of a medical device to form an imine moiety, and the imine moiety is reduced to form an amine linkage immobilizing a coating of the biomolecule on the surface. Conversely, the biomolecule can contain the amine moiety and the 2-aminoalcohol moiety can be on the surface. In another method, a biomolecule coating containing an amine moiety and a 2-aminoalcohol moiety is immobilized on the surface of a medical device, and the 2-aminoalcohol moiety is oxidized with a periodate to an aldehyde moiety which reacts with the amine moiety to crosslink the coating. A crosslinked biomaterial may be formed by combining a periodate with two or more biomolecules containing amine moieties and 2-aminoalcohol moieties to oxidize the 2-aminoalcohol moieties to aldehyde moieties, and allowing the aldehyde moieties to react with the amine moieties. When a medical device surface containing amine moieties is present with the two or more biomolecules, some of the aldehyde moieties react with the amine moieties on the medical device surface resulting in both immobilizing and crosslinking of the biomolecules on the surface.
REFERENCES:
patent: 3826678 (1974-07-01), Hoffman et al.
patent: 4521564 (1985-06-01), Solomon et al.
patent: 4600652 (1986-07-01), Solomon et al.
patent: 4613665 (1986-09-01), Larm
patent: 4642242 (1987-02-01), Solomon et al.
patent: 4720512 (1988-01-01), Hu et al.
patent: 4786556 (1988-11-01), Hu et al.
patent: 5032666 (1991-07-01), Hu et al.
patent: 5053048 (1991-10-01), Pinchuk
patent: 5077372 (1991-12-01), Hu et al.
patent: 5344455 (1994-09-01), Keogh et al.
patent: 5362852 (1994-11-01), Geoghegan
patent: 5728420 (1998-03-01), Keogh
patent: 5821343 (1998-10-01), Keogh
R.G. Dickinson et al., "A New Sensitive and Specific Test for the Detection of Aldehydes: Formation of 6-Mercapto-3-substituted-s-traizolo[4,3-b]-s-tetrazines", Chem. Commun., 1719-1720 (1970).
K.F. Geoghegan et al., "Site-Directed Conjugation of Nonpeptide Groups to Peptides and Proteins via Periodate Oxidation of a 2-Amino Alcohol. Application to Modification at N-Terminal Serine", Bioconjugate Chem., 3, 138-146 (1992).
A.S. Hoffman et al., A Covalent Binding of Biomolecules to Radiation-Grafted Hydrogels on Inert Polymer Surfaces,@ Trans. Am. Soc. Artif. Intern. Organs, 18, 10-18 (1972).
S. Holmes et al., AAmination of Ultra-high Strength Polyethylene using Ammonia Plasma,@ Composites Science and Technology, 38, 1-21 (1990).
Y. Ito et al., AMaterials for Enhancing Cell Adhesion by Immobilization of Cell-Adhesive Peptide,@ J. Biomed. Mat. Res., 25, 1325-1337 (1991).
P.H. O=Farrell, AHigh Resolution Two-Dimensional Electrophoresis of Proteins,@ J. Biol. Chem., 250, 4007-4021 (1975).
Medtronic Inc.
Naff David M.
Patton Harold R.
Woods Thomas F.
LandOfFree
Periodate oxidative method for attachment and crosslinking of bi does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Periodate oxidative method for attachment and crosslinking of bi, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Periodate oxidative method for attachment and crosslinking of bi will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2315030