Perifusion apparatus for culturing living cells and perifusion c

Details Perifusion apparatus for culturing living cells and perifusion c Perifusion apparatus for culturing living cells and perifusion c

1989-11-21

1991-04-23

Wallen, Thomas

Chemistry: molecular biology and microbiology

Miscellaneous

435289, 435290, 435313, C12M 114

Patent

active

050100149

ABSTRACT:
In a perifusion apparatus for culturing living cells, the culture medium and the gas in the culture chamber are separated from one another by a gas-permeable wall, the liquid area being entirely filled with the culture medium. Gassing takes place through the gas-permeable wall. Several culture chambers can be interconnected as plug-in modules in such a way that common means can be used for liquid circulation, gassing and optionally thermizing.

REFERENCES:
patent: 3732149 (1973-05-01), Santero
patent: 3843454 (1974-10-01), Weiss
patent: 4172013 (1979-10-01), Skoda et al.
patent: 4225671 (1980-09-01), Puchinger et al.
patent: 4228243 (1980-10-01), Iizuka
patent: 4748124 (1988-05-01), Vogler
"Hormonal Interactions in the Control of Hepatic Enzyme Levels/Primary Cultures of Hepatocytes as Model Systems for Metabolic Regulation Research", Faculty of Chem. and Pharm. of the Eberhard-Karls University in Tubingen 1980, pp. 43-48.
Perifused Monolayer Cultures of Rat Hepatocytes as an Improved in Vitro System for Studies on Ureogenesis, by Rolf Gebhardt et al., 124, 1979, pp. 349-359.
Production of Anchorage-Dependent Cells-Problems and their Possible Solutions, Biotechnology and Bioengineering, vol. XXIII, pp. 2703-2716, 1981.
Perifusion System for Isolated Cells, Analytical Biochemistry, 112, pp. 117-127 (1981).

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