Perfusive chromatography

Details Perfusive chromatography Perfusive chromatography

1993-05-10

1995-01-24

Therkorn, Ernest G.

Liquid purification or separation

With means to add treating material

Chromatography

2105021, 502402, B01D 1508

Patent

active

053840429

ABSTRACT:
Disclosed are chromatography methods and matrix geometries which permit high resolution, high productivity separation of mixtures of solutes, particularly biological materials. The method involves passing fluids through specially designed chromatography matrices at high flow rates. The matrices define first and second interconnected sets of pores and a high surface area for solute interaction in fluid communication with the members of the second set of pores. The first and second sets of pores are embodied, for example, as the interstices among particles and throughpores within the particles. The pores are dimensioned such that, at achievable high fluid flow rates, convective flow occurs in both pore sets, and the convective flow rate exceeds the rate of solute diffusion in the second pore set. This approach couples convective and diffusive mass transport to and from the active surface and permits increases in fluid velocity without the normally expected bandspreading.

REFERENCES:
patent: 3243386 (1966-03-01), Nielsen et al.
patent: 3322695 (1967-05-01), Alfray et al.
patent: 3488922 (1970-01-01), Kirkland
patent: 3505785 (1970-04-01), Kirkland
patent: 3728290 (1973-04-01), Johansson et al.
patent: 3782075 (1974-01-01), Kirkland
patent: 3855172 (1974-12-01), Iler et al.
patent: 3862030 (1975-01-01), Goldberg
patent: 4012265 (1977-03-01), Rinde
patent: 4029583 (1977-06-01), Ho Chang et al.
patent: 4070283 (1978-01-01), Kirkland
patent: 4070286 (1978-01-01), Iler et al.
patent: 4102746 (1978-07-01), Goldberg
patent: 4131542 (1978-12-01), Bergna et al.
patent: 4160728 (1979-07-01), Kirkland et al.
patent: 4169014 (1979-09-01), Goldberg
patent: 4186120 (1980-01-01), Ugelstad
patent: 4245005 (1981-01-01), Regnier et al.
patent: 4247498 (1981-01-01), Castro
patent: 4335017 (1982-06-01), Miles et al.
patent: 4336161 (1982-06-01), Rosevear et al.
patent: 4350595 (1982-09-01), Gunkel
patent: 4352884 (1982-10-01), Nakashima et al.
patent: 4384957 (1983-05-01), Crowder, III et al.
patent: 4385991 (1983-05-01), Rosevear et al.
patent: 4430451 (1984-02-01), Young et al.
patent: 4446314 (1984-05-01), Jordan
patent: 4452892 (1984-06-01), Rosevear
patent: 4477492 (1984-10-01), Bergna et al.
patent: 4483773 (1984-11-01), Yang
patent: 4496461 (1985-01-01), Leeke et al.
patent: 4501826 (1985-02-01), Mortzner et al.
patent: 4512897 (1985-04-01), Crowder, III et al.
patent: 4519909 (1985-05-01), Castro
patent: 4549584 (1985-10-01), Morin et al.
patent: 4576199 (1986-03-01), Svensson et al.
patent: 4604198 (1986-08-01), Dailey et al.
patent: 4618533 (1986-10-01), Steuck
patent: 4673734 (1987-06-01), Tayot et al.
patent: 4729834 (1988-03-01), Itoh et al.
patent: 4732687 (1988-03-01), Muller et al.
patent: 4737292 (1988-04-01), Ritacco et al.
patent: 4761232 (1988-08-01), Bright
patent: 4780113 (1988-10-01), Koslow
patent: 4986908 (1991-01-01), Stout et al.
patent: 5019270 (1991-05-01), Afeyan et al.
patent: 5037543 (1991-08-01), Maejima et al.
L. B. Bangs, "Uniform Latex Particles", a publication of Seradyn, Inc., pp. 5-9 (1987).
4 BioChromatography 1:25 (Jan/Feb. 1989), reference to oral presentation delivered at 12th Intl. Symposium on Column Liquid Chromatography, Jun. 19-24, 1988, in Washington, D.C.
Chiantore et al., 5 J. of Liquid Chromatogr. 4:643-667 (1982).
W. M. Deen, 33 AIChe J. 9:1409-1425 (1987).
J. Calvin Giddings, Dynamics of Chromatography, vol. 1 (New York:Marcell Decker, Inc.) (1965) pp. 1-323.
Giddings et al., 10 Macromolecules 2:443-449 (1977).
J. Calvin Giddings, Adv. Chromatogr. 20:217-258 (1982).
Guttman et al., 3 Macromolecules 5:681-691 (1970).
Horak et al, 117 Die Angewandte Makromolekulare Chemie 1975: 117-129 (1983).
Jacobelli et al., J. Appl. Polymer Sci. 23:927-939 (1979).
Jacobelli et al., 80 Die Angewandte Makromolekulare Chemie 1167: 31-51 (1979).
Klein et al., 14 Macromolecules 5:1411-1422 (1981).
Kun et al., J. Polymer Sci. 16:1457-1469 (1967).
Kun et al., J. Polymer Sci. 6:2689-2701 (1968).
R. Kunin, "Pore Structure of Macroreticular Ion-Exchange Resins", Ion Exch. Proc. Ind., Pap. Conf., pp. 10-15 (1970).
Lieto et al., Chemtech 46-53 (Jan., 1983).
Lloyd et al., "Polymeric Anion Exchange Columns for the HPLC Analysis of Large Biological Solutes (Proteins)", presented at the 39th Pittsburgh Conference and Exposition on Analytical Chemistry and Applied Spectroscopy, New Orleans, Louisiana, Feb. 22-26, 1988, pp. 1-11.
Lloyd et al., "Affinity and Ion Exchange Chromatographic Supports for High Performance Biological Separations", presented at the International Conference on Separations for Biotechnology, University of Reading, United Kingdom, Sep. 15-18, 1987 pp. 1-12.
Lloyd et al., "Application of Polymeric Packings in Bio-HPLC Int. Symposium on BioMedical Applications of Liquid Chromatography" Bradford, UK Mar. 23-25, 1988 pp. 1-11.
McConville et al., "Influence of Pore Size/Ionic Capacity on the Separation of Small and Large Biomolecules When Using Polymeric Anion Exchange Media", a publication of Polymer Laboratories, Inc., Amherst, Massachusetts pp. 1-9 (undated).
"High Performance Columns and Media for Today's Life Scientists", Polymer Laboratories, Ltd. publication (Shropshire, England) 1989 pp. 1-9.
Ruthven, DM Principles of Adsorption and Adsorption Processes pp. 133-141 John Wiley & Sons, N.Y., 1984.
Sederel et al., J. Appl. Polymer Sci. 17:2835-2846 (1973).
Ugelstad et al., "Effect of Additives on the Formation of Monomer Emulsions and Polymer Dispersions", Emulsion Polymerization 383-413 (1982).
van der Wiel et al., "Continuous Adsorption in Biotechnology", a preprint containing results presented at the 1986 Engineering Foundation Conference, Uppsala, Sweden pp. 1-46.
Van Kreveld et al., J. Chromatogr. 149:71-91 (1978).
Yau et al., Modern Size-Exclusion Liquid Chromatography, a Wiley-Interscience Publication (New York: John Wiley & Sons, 1978) pp. 77-95.
Kopaciewicz et al., J. Chromatogr. 409:111-124 (1987).
Rodrigues et al., (1982) AlChE Journal 28(4):541-596.
"Nonlinear Adsorption, Reaction, Diffusion and Intraparticle Convection Phenomena in Flow Systems"pp. 147-180 in Residence Time Distribution Theory in Chemical Engineering, A. Petho and R. D. Noble, eds. Proc. Summer Sch., 1982, Rodrigues.
Rodrigues et al., (1984) Chem. Eng. Commun 27:327-337.
Altenberger et al., "On the Theory of Self-diffusion in a Polymer Gel", J. Chem. Phys., 80:2208-2213 (1984).
Arnold et al., "Analysis of Affinity Separations I: Predicting the Performance of Affinity Absorbers," Chem. Eng. Journal, 30:B9-B23 (1985).
Arnold et al., "Analysis of Affinity Separations II: The Characterizations of Affinity Columns By Pulse Techniques," Chem. Eng. Journal, 30:B25-36 (1985).
Attwood, T. K., "Electron Microscopy of Beaded Agarose Gels", Biopolymers, 27:201-212 (1988).
Aubert et al., "Low Density Microcellular Polystyrene Foams", Polymer, 26:2047-54 (1985).
Bartholin, "Styrene-Divinylbenzens Copolymers, 3. Revisited IR Analysis", Macromol. Chem., 182:2075-2085 (1981).
Bergstrom et al., "Sample Loading Capacity on Monobeads Ion Exchangers," 30th Colloquim: Protides of Biological Fluids, (1982) pp. 647-651.
Bergstrom et al., "Recommendations for Obtaining Optimal Separations on Monobead Ion Exchangers," 30th Colloquium: Protrides of Biological Fluids, (1982) pp. 641-646.
Blanche et al., "Chromatography System For Use in the Development and Scaleup of Pharmaceutical Biomolecules," Amer. Laboratory, (1988) pp. 1-6.
Boschetti, E, "Polyacrylamide Derivatives to The Service of Bioseparations", Journal of Biochemical and Biophysical Methods, 19: 21-36 (1989).
Boersma-Klein et al., "The Evaluation in Time Domain of Mass Transfer Parameters from Chromatographic Peaks," Chem. Engineering Science, 34: 959-69 (1979).
Brandt, S., "Resolving Chiral Synthesis Problems," Performance Chemicals, (1987) pp. 1-4.
Brandt et al., "Membrane-Based Affinity Technology for Commerical Scale Purifications," Bio/Technology, 6:779-782 (1988).
Brown et al., "Solute Diffusion in Hydrated Polymer Networks," (1975) pp. 12-21.
Burton et al., "Separation of Proteins by Reversed-Phased High-Performance Liquid Chromatograph

Affiliated with

Also associated with

Rating

Perfusive chromatography does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Perfusive chromatography, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Perfusive chromatography will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1466405