Perfusion system and a method for the large scale production of

Details Perfusion system and a method for the large scale production of Perfusion system and a method for the large scale production of

1994-12-13

1998-02-17

Lankford, Blaine

Chemistry: molecular biology and microbiology

Virus or bacteriophage, except for viral vector or...

435239, 435174, 435176, 435177, 435364, C12N 702, C12N 701

Patent

active

057190518

ABSTRACT:
The invention resides in a matrix, i.e. in a carrier material, with human or animal cells adherently bound thereto, the cells being infected with virus. It has shown that surface-dependent cells suitable for virus propagation remain adherently bound to a matrix even in the virus-infected state, continuously produce virus antigen over relatively long periods of time and deliver them into the culture medium. For producing TBE virus antigen by growing tick-borne encephalitis (TBE) virus in cell cultures, a surface-dependent permanent cell line, preferably the Vero cell line ATCC CCL 81, is inoculated with TBE virus, and the cells are kept bound to carriers in a non-lyric serum-free system while maintaining the cell growth, so as to maintain antigen formation, whereupon the antigen-containing medium is separated form the carrier-bound cells and, in a known manner, is processed to a galencially acceptable preparation by concentration, inactivation and purification.

REFERENCES:
patent: 4072565 (1978-02-01), Weiss et al.
patent: 4525349 (1985-06-01), Montagnon et al.
patent: 4699785 (1987-10-01), Pederson
patent: 5391491 (1995-02-01), Mundt et al.
J. Buzek, Separation of Time-defined Avian Myeloblastosis Virus(AMV) Using Column Cultivation of Leukaemic Myeloblasts, Institute of Organic Chemistry and Biochemistry, pp. 97-100 (Aug. 20, 1975).
Greenwalt, et al., A New Method for Preparing Buffy Coat-Poor Blood, Tranfusion, vol. 2 pp. 221-229 (1962).
Yale Rabinowitz, Separation of Lymphocytes, Polymorphonuclear Leukocytes and Monocytes on Glass Columns, Including Tissue Culture Observations, Blood, vol. 23, No. 6, pp. 811-828 (Jun. 1964).
Hoke, et al, "Protection Against Japanese Encephalitis By Inactivated Vaccines," New England Journal of Medicine, 319:608-614, (1988).
Kunz, et al. "Immunogenicity and Reactogenicity of a Highly Purified Vaccine Against Tick-bone Encephalitis", Journal of Medical Virology, 6:103-109, (1980).
Jackwood et al. "Replication of Infectious Bursal Disease Virus in Continuous Cell Lines," Avian Diseases, 21(2): 370-375 (1986).
Florentine et al. Dev. Biol. Stand. 60: 421-430 (1985).
Widell et al. J. Virol. Methods 8: 63-71 (1984).
Griffiths et al. Develop. Biol. Standard 50: 103-110 (1982).
Reuveny et al. Dev. Biol. Stand., 60: 243-253 (1985).
Lazar et al. Dev. Biol. Stand., 60: 457-465 (1985).
caij et al. Arch Virol, 105: 113-118 (1989).
Buzek, Folio Biol. 22(2) 1976, pp. 97-100 (Biosis ABS).
Poindinger et al. J. of Gen Virology. 1991 pp. 573-578.
Smith et al. J. of Clinical Mocrobiology 1984, pp. 267-272.
Deryabin et al. VOPR Virusol (2), 1975 pp. 235-238, (See Biosis Abstract).

Affiliated with

Also associated with

Rating

Perfusion system and a method for the large scale production of does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Perfusion system and a method for the large scale production of , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Perfusion system and a method for the large scale production of will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1783423