Performing an enzyme-catalyzed reaction

Details Performing an enzyme-catalyzed reaction Performing an enzyme-catalyzed reaction

1989-08-04

1991-11-05

Lilling, Herbert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 18, 435 19, 435 23, 435 39, 435135, C12Q 134, C12Q 144, C12Q 100, C07D31182

Patent

active

050631497

ABSTRACT:
A process for performing an enzyme-catalyzed reaction in a reaction mixture comprising two phases, a first aqueous phase containing an enzyme which is not accessible and a second substantially non-aqueous phase containing a non-water miscible solvent wherein a compound derived from fluorescein or a substituted fluorescein is present for the purpose of measuring aqueous pH by monitoring spectroscopic properties. Novel compounds derived from fluorescein or a substituted fluorescein which may be used in the process and a method for producing some of the novel compounds are also claimed.

REFERENCES:
patent: 4267273 (1981-05-01), Smith
patent: 4711245 (1987-12-01), Higgins et al.
Biotech Data Base 88-09718, Roberts et al. 9996F; Biochem Soc. Trans (1988) 16, 3, 273-74.
Chem. Abs. 85:144976(a), Jul. 5, 1976, Asahi et al, J7677432.
Chem. Abs. 85:144978(c), Jul. 5, 1976, Sasage et al, J7677434.

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